委中穴齐刺法促进腰椎间盘突出模型大鼠多裂肌修复的机制研究  

Mechanism Study on Promoting Multifidus Muscle Repair in Rats with Lumbar Disc Herniation by Triple Needling at Weizhong(BL40)

作　　者：许晶晶 吴耀持[2] 郑明岳[2] 李艳 Xu Jingjing;Wu Yaochi;Zheng Mingyue;Li Yan(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学,上海201203 [2]上海交通大学医学院附属第六人民医院,上海200233

出　　处：《中国中医急症》2024年第10期1728-1733,共6页Journal of Emergency in Traditional Chinese Medicine

基　　金：上海市第六人民医院院级课题(ynhg202327)。

摘　　要：目的 探寻委中穴齐刺法促进腰椎间盘突出模型大鼠多裂肌修复的作用机制。方法 选用24只6~8周龄雄性SD大鼠,随机分为空白组、模型组、普通针刺组、齐刺组,每组6只。除空白组外其余3组均建立自体髓核移植的椎间盘突出大鼠模型,造模成功后3 d开始针刺干预,普通针刺组普通针刺委中穴,齐刺组采用三针齐刺委中穴,在造模前、造模1 d、造模3 d、针灸7、14、21 d分别进行大鼠后肢机械缩足痛阈检测。HE染色观察多裂肌病理形态,Western blotting、Real-time PCR检测多裂肌血管内皮生长因子(VEGF)、成肌分化蛋白(MyoD)、成对框基因(Pax7)水平。结果 造模后大鼠机械痛阈明显降低,HE病理染色结果提示模型组肌纤维萎缩,细胞核内移。经针刺治疗21 d肌纤维横截面积增大,机械痛阈显著升高,齐刺组优于普通针刺组(P <0.05),委中穴齐刺干预后VEGF、MyoD、Pax7水平较模型组升高(P <0.05)。结论 委中穴齐刺法能够提高腰椎间盘突出模型鼠痛阈,促进多裂肌血管新生、提高肌纤维修复能力。Objective:To explore the mechanism of triple needling at Weizhong(BL40)to promote the repair of multifidus muscle in rats with lumbar disc herniation.Methods:Twenty-four male SD rats aged 6-8 weeks were randomly divided into the blank group,model group,ordinary acupuncture group and triple needling group,with 6 rats in each group.In addition to the blank group,the other three groups established a rat model of intervertebral disc herniation with autologous nucleus pulposus transplantation.Acupuncture intervention started 3 days after suc-cessful modeling.The common acupuncture group was treated with common acupuncture at Weizhong(BL40),and the triple needling group was treated with triple acupuncture at Weizhong(BL40).The mechanical paw withdrawal threshold of the hind limbs of the rats was detected before modeling,1 day after modeling,3 days after modeling,7 days after acupuncture,14 days after acupuncture,and 21 days after acupuncture.HE staining was used to observe the pathological morphology of multifidus muscle.Western blotting and real-time PCR were used to detect the lev-els of VEGF,MyoD and Pax7 in multifidus muscle.Results:After modeling,the mechanical pain threshold of rats was significantly reduced.The results of HE pathological staining showed that the muscle fibers in the model group were atrophied,and the nucleus moved inward.After 21 days of acupuncture treatment,the cross-sectional area of muscle fibers increased,and the mechanical pain threshold increased significantly.The triple needling group was better than the ordinary acupuncture group(P<0.05).The levels of VEGF,MyoD and Pax7 were higher than those in the model group(P<0.05).Conclusion:Triple needling at Weizhong(BL40)can improve the pain threshold of lumbar disc herniation model rats,promote the angiogenesis of multifidus muscle and improve the abil-ity of muscle fiber repair.

关 键 词：腰椎间盘突出 委中穴 齐刺法 多裂肌 肌卫星细胞 大鼠 

分 类 号：R285.5[医药卫生—中药学]