乌汶伯克霍尔德菌P5和格氏假单胞菌RP22促杉木生长机制探究  

Mechanism of Burkholderia ubonensis P5 and Pseudomonas grimontii RP22 to Promote Growth of Chinese fir

作　　者：陈详腾 魏书蒙 焦如珍[1] 董玉红[1] CHEN Xiang-teng;WEI Shu-meng;JIAO Ru-zhen;DONG Yu-hong(Research Institute of Forestry,Chinese Academy of Forestry,State Key Laboratory of Efficient Production of Forest Resources,Beijing 100091,China)

机构地区：[1]林木资源高效生产全国重点实验室,中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学研究》2024年第5期33-45,共13页Forest Research

基　　金：林业和草原科技成果国家级推广项目“高效多功能菌剂在杉木大田育苗中的推广应用”(2023133101)。

摘　　要：[目的]本文拟探究乌汶伯克霍尔德氏菌P5和格氏假单胞菌RP22对杉木幼苗生长、基因表达和土壤性质的影响,初步揭示2种溶磷菌促杉木生长机制。[方法]以6个月苗龄的杉木实生苗为试验材料,将配制好的P5、RP22及P5+RP22菌剂通过根灌方式施加,每月施加一次,持续3个月。3个月后对幼苗和土壤进行取样,测定幼苗的生长生理指标和土壤指标;基于Illumina HiSeq 4000测序平台对幼苗进行转录组分析,并筛选杉木幼苗的促生基因。[结果]与对照组(CK)相比,3种菌剂处理均促进杉木幼苗的生长。3种菌剂对杉木幼苗的促生生理机制包括:PSB提高了杉木幼苗的叶绿素、可溶性糖、可溶性蛋白等生理指标及磷含量;同时,增加了土壤有效磷、速效钾、全磷养分含量。转录组结果表明,3种菌剂处理后杉木幼苗的差异表达基因(DEGs)均显著富集在代谢物合成、苯丙烷生物合成、亚油酸代谢、α亚麻酸代谢和光合作用通路。对这些通路的深入分析发现,PAL、COMT、4CL、LOX1_5、AOS、AOC等基因在P5菌剂处理组显著上调;PAL、TOGT1、CYP73A、LOX1_5、psbS、psaF等基因在RP22菌剂处理组显著上调;LOX1_5、psbO、petH、psaF等基因在P5+RP22菌剂处理组显著上调,表明3种PSB菌剂对杉木幼苗的促生机制是多个代谢通路相互协调的结果。[结论]3种菌剂能通过调控杉木幼苗的生理、提高土壤养分含量和上调表达苯丙烷生物合成、亚油酸代谢、光合作用等通路中的基因,从而促进杉木幼苗生长。[Objective]To investigate the effects of Burkholderia ubonensis P5 and Pseudomonas grimontii RP22 on the growth of Chinese fir seedlings for revealing the mechanism of the two types of PSBs in promoting growth of Chinese fir.[Method]Based on the six-month-old live Chinese fir seedling,the formulated P5,RP22 and P5+RP22 fungicides were applied by root irrigation once a month for 3 months.Seedlings and soil were sampled after 3 months to determine the growth properties,physiological properties of seedlings and soil properties;transcriptome analysis of seedlings was performed based on Illumina HiSeq 4000 sequencing platform and screening of Chinese fir seedling growth-promoting genes.[Result]Compared with the control group(CK),microbial agent addition of all three treatments promoted the growth of Chinese fir seedlings.The physiological mechanisms of the three treatments on the growth of Chinese fir seedlings included:PSB increased the physiological indexes such as chlorophyll,soluble sugars,soluble proteins,and the phosphorus content of Chinese fir seedlings;and at the same time,it increased the content of active phosphorus,active potassium,and total phosphorus of soil.Transcriptome results indicated that differentially expressed genes(DEGs)in Chinese fir seedlings after three treatments were significantly enriched in metabolite synthesis,phenylpropane biosynthesis,linoleic acid metabolism,α-linolenic acid metabolism,and photosynthesis pathways.The pathways analysis revealed that PAL,COMT,4CL,LOX1_5,AOS and AOC genes were significantly up-regulated in the P5 treatment;PAL,TOGT1,CYP73A,LOX1_5,psbS and psaF genes were significantly up-regulated in the RP22 treatment;and LOX1_5,psbO,petH and psaF genes were significantly up-regulated in the P5+RP22 treatment.It indicated that the growth-promoting mechanism of the three treatments on Chinese fir seedlings was the result of multiple metabolic pathways coordinating with each other.[Conclusion]The three fungicides treatments can promote the growth of Chinese fir seedling

关 键 词：杉木幼苗 溶磷菌 转录组 差异表达基因 促生机理 

分 类 号：S714.3[农业科学—林学]