GATA 1和BCL-xL在骨髓增生异常综合征患者外周血单个核细胞中的表达研究  

Study on the expression of GATA1 and BCL-xL in peripheral blood cells of patients with myelodysplastic syndrome

作　　者：马思飞 杨红梅[1] MA Sifei;YANG Hongmei(Changzhou Central Blood Bank,Blood Transfusion Laboratory,Jiangsu 213003)

机构地区：[1]常州市中心血站输血研究室,江苏213003

出　　处：《交通医学》2024年第4期331-334,340,共5页Medical Journal of Communications

基　　金：常州市中心血站课题(xz202402)。

摘　　要：目的:探讨珠蛋白转录因子1(globin transcription factor 1,GATA 1)和B细胞淋巴瘤因子(B-cell leukemia/lymphoma xL,BCL-xL)在骨髓增生异常综合征(myelodysplastic syndromes,MDS)患者外周血单个核细胞中的表达以及Notch信号通路相关机制。方法:以36例MDS患者为MDS组,20例定期献血的健康人为对照组。采用实时荧光定量PCR法检测外周血单个核细胞中GATA 1、BCL-xL、Notch 1及其下游分子HES 1和Herp 2的表达,分析GATA 1和BCL-xL的表达与MDS患者血小板计数和血红蛋白的相关性,采用流式细胞术分析外周血单个核细胞的凋亡。结果:与对照组比较,MDS患者外周血单个核细胞中GATA 1和BCL-xL mRNA表达量明显降低(P<0.05),而Notch 1、HES 1、Herp 2 mRNA表达量显著增加(P<0.05)。GATA 1 mRNA表达水平与MDS患者血小板计数呈正相关(r=0.7575,P=0.0181)。与对照组比较,凋亡细胞比例增高(P<0.01)。结论:MDS患者GATA 1和BCL-xL mRNA表达下降,Notch信号通路下游因子HES 1和Herp 2增高,可能是导致细胞凋亡显著增加的原因。Objective:To investigate the expression of globin transcription factor 1(GATA 1)and B-cell leukemia/lymphoma xL(BCL-xL)in peripheral blood cells of patients with myelodysplastic syndromes(MDS)and explore the mechanism related to Notch signaling pathway.Methods:A total of 36 MDS patients were selected as the MDS group and 20 healthy people who donated blood regularly were selected as the control group.Real-time fluorescence quantitative PCR was used to detect the expression of GATA 1,BCL-xL,neurogenic gene Notch homologous protein 1(Notch 1)and its downstream molecules HES 1 and Herp 2 in peripheral blood cells.The correlation between the expression of GATA 1,BCL-xL and platelet count,hemoglobin in MDS patients was analyzed.The apoptosis of peripheral blood cells was observed by flow cytometry.Results:Compared with the control group,the mRNA levels of GATA 1 and BCL-xL were significantly decreased in peripheral blood of MDS patients(P<0.05),while the mRNA levels of Notch 1,HES 1 and Herp 2 were significantly increased(P<0.05).Moreover,the expression of GATA 1 mRNA was positively correlated with the platelet count in MDS patients(r=0.7575,P=0.0181).Compared with the control group,the apoptotic cells increased in the MDS group(P<0.01).Conclusion:The expression of GATA 1 and BCL-xL decreased,and the expression of Notch 1,HES 1 and Herp 2 increased in MDS patients,which may be the cause of significant increase in cell apoptosis.

关 键 词：骨髓增生异常综合征 珠蛋白转录因子1 B细胞淋巴瘤因子 NOTCH信号通路 凋亡 

分 类 号：R551.3[医药卫生—血液循环系统疾病]