Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes  

作　　者：Xinmeng Li Weixiao Ding Shujun Wang Luyu Yang Qingqing Yu Changji Xiao Guangbo Chen Lei Zhang Shanyue Guan Dongping Sun 

机构地区：[1]Institute of Chemicobiology and Functional Materials,School of Chemistry and Chemical Engineering,Nanjing University of Science and Technology,200 Xiao Ling Wei,Nanjing 210094,China [2]Department of Blood Transfusion,Jinling Hospital,Nanjing University School of Medicine,Nanjing 210002,China [3]Obstetrics and Gynaecology Department,Peking University First Hospital,Peking University,Beijing 100034,China [4]Center for Advancing Electronics Dresden(Cfaed)and Faculty of Chemistry and Food Chemistry,Technische Universitat Dresden,01062 Dresden,Germany [5]Key Laboratory of Photochemical Conversion and Optoelectronic Materials,Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,China

出　　处：《Cyborg and Bionic Systems》2023年第1期368-378,共11页类生命系统（英文）

基　　金：support from the National Natural Science Foundation of China(51873087,51803092,and 81801839);the Youth Innovation Promotion Association of Chinese Academy of Sciences(No.2019027);the Director Foundation of the Technical Institute of Physics and Chemistry,Chinese Academy of Sciences.

摘　　要：The liver is the hub of human metabolism and involves many diseases.To better work on the mechanism and treatment of liver diseases,it is of particular interest to design 3-dimensional scaffolds suitable for culturing hepatocytes in vitro to simulate their metabolic and regenerative abilities.In this study,sulfated bacterial cellulose(SBC)was prepared as the building block of cell scaffolds,motivated by the anionic nature and 3-dimensional structure of hepatic extracellular matrix,and its reaction condition for sulfate esterification was optimized by changing the reaction time.The analysis and study of the microscopic morphology,structure,and cytocompatibility of SBCs showed that they possess good biocompatibility and meet the requirements for tissue engineering.Next,SBC was mixed with gelatin for composite scaffolds(SBC/Gel)for culturing hepatocytes by homogenization and freeze-drying methods,whose physical properties such as pore size,porosity,and compression properties were compared with gelatin(Gel)scaffolds as the control group,and the cytological activity and hemocompatibility of the composite scaffolds were investigated.The results showed that the SBC/Gel composite has better porosity and compression properties,as well as good cytocompatibility and hemocompatibility,and could be applied to 3-dimensional culture of hepatocytes for drug screening or liver tissue engineering.

关 键 词：METABOLISM compatibility POROSITY 

分 类 号：R318[医药卫生—生物医学工程]