Genetic Polymorphisms of 22 Novel Autosomal Short Tandem Repeat Loci in Sierra Leone Population  

作　　者：Yi Liu Chudong Wang Dan Wen Weifeng Qu Hao Xing Hongtao Jia Ruyi Xu Xuan Tang Siqi Chen Jienan Li Guanlin Li Lagabaiyila Zha Yong Wang 

机构地区：[1]Department of Forensic Medicine,School of Basic Medical Sciences,Central South University,Changsha,China [2]Department of Clinical Laboratory,First Affiliated Hospital of Zhengzhou University,Zhengzhou,China [3]China-Africa Research Center of Infectious Diseases,Central South University,Changsha,China

出　　处：《Journal of Forensic Science and Medicine》2024年第3期209-213,I0001-I0008,共13页法庭科学与法医学杂志（英文）

基　　金：This work was supported by the Key Program of National Natural Science Foundation of China(NSFC)(grant number 82230064);the General Program of National Natural Science Foundation of China(NSFC)(grant number 82271928).

摘　　要：Background:Exploring and identifying novel alleles of noncombined DNAIndex System(CODIS)short tandem repeat(STR)loci in different ethnic groups is important for the establishment of forensic reference databases and study of population genetics.Aim:This study is aimed to explore the genetic polymorphism of 22 non-CODIS autosomal STR loci(D6S477,D18S535,D19S253,D15S659,D11S2368,D20S470,D1S1656,D22-GATA198B05,D8S1132,D4S2366,D21S1270,D13S325,D9S925,D3S3045,D14S608,D10S1435,D12S391,D7S3048,D17S1290,D5S2500,D2S1338,and D16S539)in Sierra Leone population and analyze the population genetic relationships in comparison with otherpopulations.Materialsand ethods:The amples of a total of 495 unrelated individuals(274 females and 221 males)from Sierra Leonewere examined by the Microreader^(TM)23SPID System,and their genetic polymorphisms and associated forensic parameters were calculated.The genetic relationships between Sierra Leonepopulation and other populations were evaluated as well.Results:Atotal of 287 alleles were observed with allelic frequencies ranging from 0.001 to 0.399.The cumulative power of discrimination(CPD)of the 22 autosomal STR loci was 0.99999999999999999999999999999538.The cumulative probability of exclusion(CPE)of the 22 autosomal STR loci was 0.9999998514(CPEdous)and 0.9999999999826(CPEtrios).All of the STR loci reached the Hardy–Weinberg equilibrium after Bonferroni correction.The population genetics analysis results demonstrated that Sierra Leone population exhibited distinctive genetic characteristics compared to those of East Asian populations and it had relatively close genetic distances to the Uygur population.Conclusion:The results of this study could enrich the forensic databases with Sierra Leone population.The 22 STR loci are highly polymorphic and could be used for forensic practice and population genetics studies.

关 键 词：Genetic polymorphisms Microreader™23SP ID System short tandem repeat Sierra Leone 

分 类 号：Q343.2[生物学—遗传学]