新型磁电催化纳米粒子的活性氮释放与抗菌性能研究  

Novel Magnetoelectric Catalytic Nanoparticles:RNS Release and Antibacterial Efficiency

作　　者：张志民 葛敏[1] 林翰 施剑林[1,2] ZHANG Zhimin;GE Min;LIN Han;SHI Jianlin(State Key Laboratory of High Performance Ceramics and Superfine Microstructures,Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200050,China;Center of Materials Science and Optoelectronics Engineering,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院上海硅酸盐研究所,高性能陶瓷和超微结构国家重点实验室,上海200050 [2]中国科学院大学材料科学与光电技术学院,北京100049

出　　处：《无机材料学报》2024年第10期1114-1124,I0002,I0003,共13页Journal of Inorganic Materials

基　　金：国家自然科学基金(52372276)。

摘　　要：相比于功能单一且易催生细菌耐药性的抗生素等药物,具有催化活性的无机纳米功能材料凭借自身对感染微环境(弱酸、高H_(2)O_(2)含量)或外部物理刺激(激光、超声)的高响应性和广谱杀菌等优势,在致病菌感染的治疗中占据愈发重要的地位。然而,感染微环境酸性微弱且不稳定,光、声信号功率密度过高会对人体细胞造成伤害,而诸如交变磁场等非侵入性、高组织穿透性和易于远程控制的信号类型及其介导的磁电催化在抗菌中的应用尚未见报道。本研究将基于磁致伸缩-压电催化效应的交变磁场响应性纳米催化策略应用于抗菌,并使用含氮基团L-精氨酸(LA)修饰CoFe_(2)O_(4)-BiFeO_(3)磁电纳米颗粒(BCFO)表面,以实现磁电响应可控释放强杀菌物种活性氮(RNS)。在交变磁场中,BCFO同时产生羟基自由基(·OH)和超氧阴离子(·O_(2)^(-))两种活性氧(ROS),前者与LA反应产生一氧化氮(NO),后者与NO反应生成RNS物种过氧亚硝酸根(ONOO^(-))。作为高活性的硝化和氧化剂,ONOO^(-)可在生物友好的交变磁场下展现出比ROS更强的抗菌能力。本研究证实BCFO能产生ONOO^(-),并发挥更强的杀菌功效。这一研究不仅将磁电纳米催化医学策略用于抗菌,还通过ROS向RNS转变显著提升了材料的抗菌性能。Compared with antibiotics and other drugs with poor functionalities and risk to induce bacterial resistance,inorganic functional nanomaterials with catalytic activity occupy an increasingly important position in the treatment of pathogenic infections by advantages of high response to the infected microenvironment(e.g.weak acid,high H_(2)O_(2) concentration)or external physical stimuli(e.g.laser,ultrasound)and broad-spectrum sterilization.However,the acidic infection microenvironment is weak and unstable,and light or sound signals with high power density will cause damage to human cells.In addition,antimicrobial applications of alternative magnetic field(AMF),a non-invasive signal type with high tissue penetration,convenience to be remotely controlled,and effective magnetoelectric catalysis based on AMF have not been reported.In this study,an AMF-responsive nanocatalytic strategy based on the magnetostrictive-piezoelectric catalytic effect was applied to antibacterial research,and the surface of CoFe_(2)O_(4)-BiFeO_(3) magnetoelectric nanoparticles(BCFO)was modified with the nitrogen-containing group L-arginine(LA)to achieve a magneto-electric responsive controlled release of powerful bactericide reactive nitrogen species(RNS).In AMF,BCFO simultaneously generates reactive oxygen species(ROS)hydroxyl radical(·OH)and superoxide anion(·O_(2)^(-)).The former reacts with LA to release nitric oxide(NO),and the latter combines with NO to produce peroxynitrite(ONOO^(-)),a typical RNS.As a highly active nitrification and oxidation agent,ONOO^(-)could exhibit stronger antibacterial activity than ROS under biofriendly AMF.Successful production of ONOO^(-)and achievement of stronger bactericidal efficiency were validated in this study.This work not only applies magnetoelectric nanocatalysis for antibacterial purposes,but also significantly improves the antibacterial ability through the conversion of ROS to RNS.

关 键 词：纳米催化医学 磁电响应 活性氮 抗菌 

分 类 号：TB34[一般工业技术—材料科学与工程] R613[医药卫生—外科学]