基于声动力和类酶活性的铌基涂层:抗菌及促进细胞增殖与分化  

Sonodynamic and Enzyme-like Activities of Niobium-based Coatings:Antimicrobial,Cell Proliferation and Cell Differentiation

作　　者：张淑敏 奚晓雯 孙磊 孙平 王德强[1] 魏杰[1] ZHANG Shumin;XI Xiaowen;SUN Lei;SUN Ping;WANG Deqiang;WEI Jie(Key Laboratory of Preparation and Application of Ultrafine Materials,Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai 200237,China;Department of Orthopedics,Shanghai Eighth People's Hospital,Shanghai 200235,China)

机构地区：[1]华东理工大学材料科学与工程学院,超细材料制备与应用教育部重点实验室,上海200237 [2]上海市第八人民医院骨科,上海200235

出　　处：《无机材料学报》2024年第10期1125-1134,共10页Journal of Inorganic Materials

基　　金：国家自然科学基金(32171340)。

摘　　要：目前感染性骨缺损修复仍然是临床难题。本研究采用微弧氧化结合水热合成方法在纯铌表面原位构建氧化铌/硫化铁异质结涂层MN@FS。结果表明,该异质结不仅具有类酶活性,而且在超声作用下具有声动力性能。在模拟细菌感染的酸性条件下,超声可触发异质结声动力,并增强类氧化酶活性,产生多种活性氧以协同抗菌/清除细菌生物膜,其抑菌率和清除率分别为98.57%和91.43%。在模拟生理条件下,超声可触发异质结,增强其类抗氧化酶活性,清除活性氧,缓解氧化应激,促进骨髓间充质干细胞(rBMSCs)的增殖与成骨分化。综上,该涂层材料在感染性骨修复方面具有广阔的应用前景。Currently,repair of infectious bone defects is still a clinical serious challenge.Here,a heterojunction coating of niobium oxide on pure niobium surface by using microarc oxidation and hydrothermal treatment was prepared.The results showed that the obtained heterogeneous junctions exhibited synchronously enzyme-like and ultrasonic dynamic properties triggered by ultrasound treatment,especially in acidic microenvironment of mimic bacterial infection.Under these key conditions,oxidase-like activity of the heterogeneous junctions was enhanced,generating several reactive oxygen species which can kill bacteria and remove their biofilm,with inhibition and clearance rates were 98.57%and 91.43%,respectively.Under simulated physiological conditions,ultrasound enhanced antioxidant-like enzyme activity of this kind heterogeneous junction,thereby scavenging reactive oxygen species,alleviating oxidative stress,and promoting proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells(rBMSCs).In conclusion,the obtained niobium-based coatings with sonodynamic and enzyme-like activities have profound potential application prospects in infectious bone repair.

关 键 词：异质结 声动力治疗 类酶活性 抗菌 成骨分化 

分 类 号：TG174[金属学及工艺—金属表面处理]