哺乳动物细胞悬浮培养技术及其应用  

Suspension Culture Technology of Mammalian Cells and Its Application

作　　者：陈铭杰 毕海洋 马懿昭 章圣华 张艳艳 张燕茹 杨松柏 周晓龙 乔自林[2] 李向臣 武永淑 CHEN Mingjie;BI Haiyang;MA Yizhao;ZHANG Shenghua;ZHANG Yanyan;ZHANG Yanru;YANG Songbai;ZHOU Xiaolong;QIAO Zilin;LI Xiangchen;WU Yongshu(Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province,Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet Technology,Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management,China-Australia Joint Laboratory for Animal Health Big Data Analytics,College of Animal Science and Technology&College of Veterinary Medicine of Zhejiang A&F University,Hangzhou 311300,China;Gansu Technology Innovation Center of Animal Cell,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]浙江农林大学动物科技学院•动物医学院,浙江省畜禽绿色生态健康养殖应用技术研究重点实验室,动物健康互联网检测技术浙江省工程实验室,动物医学与健康管理浙江省国际科技合作基地,中澳动物健康大数据分析联合实验室,杭州311300 [2]西北民族大学,甘肃省动物细胞技术创新中心,兰州730030

出　　处：《中国细胞生物学学报》2024年第9期1711-1718,共8页Chinese Journal of Cell Biology

基　　金：西北民族大学生物医学研究中心开放基金(批准号:BRC-KF202304)资助的课题。

摘　　要：悬浮培养技术是将细胞悬浮于培养液中,用于病毒增殖或蛋白表达的技术。该技术具有细胞培养规模大、目的产物表达量高、表达产物质量好和利于产业化等优点。当前,多种细胞已通过选择合适的生物反应器、使用微载体和无血清培养基等方法被成功悬浮培养,用于病毒灭活疫苗、重组蛋白和细胞治疗等领域。该文阐述了哺乳动物细胞悬浮培养技术以及其在病毒灭活疫苗、重组蛋白和细胞治疗等方面的应用,以期为悬浮培养技术的发展和应用提供一定参考。Suspension culture technology involves suspending cells in a culture medium,which is utilized for virus propagation or protein expression.This technology is often utilized for purposes such as virus proliferation or protein expression.It has several advantages,including scalability for scale-large cell culture,high expression levels of target products,high quality of products and advantage for industrial application.Various cell types have been successfully adapted to suspension cultures through the use of suitable bioreactors,microcarriers,and serum-free media.These cells are currently being utilized in a range of applications,including inactivated virus vaccine production,recombinant proteins and cell therapy.This review provides an in-depth analysis of mammalian cell suspension culture domestication technology and its applications in the fields of inactivated virus vaccines,recombinant proteins,and cell therapy.By providing insights into the development and application of suspension culture technology,this review aims to serve as a valuable resource for researchers in this field.

关 键 词：哺乳动物细胞 生物反应器 微载体 无血清培养基 生物制品 

分 类 号：Q813.11[生物学—生物工程]