一测多评法同时测定悬钩木中3种化学成分  

Determination of 3 Chemical Components in Rubus sachalinensis Lévl.by Quantitative Analysis of Multi-component by Single Marker

作　　者：马志良 李竣[1] 杜连平 Ma Zhiliang;Li Jun;Du Lianping(School of Pharmaceutical Sciences,South-Central MINZU University,Wuhan,430074,Hubei,China;Qinghai Tibetan Medicine Research Institute,Xining 810016,Qinghai,China)

机构地区：[1]中南民族大学药学院,湖北武汉430074 [2]青海省藏医药研究院,青海西宁810016

出　　处：《绿色科技》2024年第18期252-256,共5页Journal of Green Science and Technology

基　　金：湖北省重点研发计划项目(编号:2022BCA053);湖北省科技创新人才及服务专项项目(国际科技合作类)(编号:2022EHB053)。

摘　　要：建立一测多评法(QAMS)同时测定悬钩木中3种成分的含量。运用高效液相色谱法(HPLC),以安捷伦SB-Aq-C18为色谱柱(250 mm×4.6 mm, 5μm),以乙腈-0.1%磷酸水溶液(17∶83,V/V)为流动相;柱温30℃,检测波长360 nm,流速1.0 mL/min;进样量10μL。以咖啡酸为内参物,计算鞣花酸、金丝桃苷的相对较正因子(f),并测定其含量。结果表明:咖啡酸、鞣花酸、金丝桃苷分别在0.18~1.85μg(r=0.9997),0.06~0.62μg(r=0.9998),0.03~0.30μg(r=0.9993)范围内与峰面积线性关系良好;平均加样回收率为97.66%~98.23%,RSD为1.77%~2.25%(n=9),均小于3.0%。指出了所建立的一测多评法可用于悬钩木中3种成分含量的同时测定,且方法简单、有效、结果准确。Three chemical constituents in Rubus sachalinensis are simultaneously determined by using quantitative analysis of multi-components by single-marker(QAMS).An high performance liquid chromatography(HPLC)method was used.The determination was performed on SB-Aq-C 18(250 mm×4.6 mm,5μm)with acetonitrile-0.1%phosphoric acid(17∶83,V/V)as the mobile phase.The column temperature,detection wavelength,flow rate and injection volume were 30℃,230 nm,1.0 mL/min,10μL,respectively.With caffeic acid as the internal standard,the relative correction factors of ellagic acid and hyperoside were calculated,and then the content determination were carried out.The standard curves of caffeic acid,ellagic acid and hyperoside all showed good linearity within 0.18~1.85μg(r=0.9997),0.062~0.62μg(r=0.9998),0.03~0.30μg(r=0.9993)respectively.The average sample recovery range was 97.66%~98.23%.RSD range was 1.77%~2.25%(n=9)with the RSD<3.0%.The established QAMS method can be used for simultaneously determining the contents of 3 components in Tibetan medicine Rubus sachalinensis,and the method is simple,effective and accurate.

关 键 词：一测多评法 悬钩木 咖啡酸 鞣花酸 金丝桃苷 含量测定 

分 类 号：R29[医药卫生—民族医学]