一测多评法同时测定大活络丸中16种挥发性成分含量  

Simultaneous determination of 16 volatile components in Dahuoluo Pills by QAMS

作　　者：蔺瑞丽 刘康康 靳婉君 赖晶 倪琳 张明童 李冬华 LIN Ruili;LIU Kangkang;JIN Wanjun;LAI Jing;NI Lin;ZHANG Mingtong;LI Donghua(Gansu Institute for Drug Control,Lanzhou 730000,China;School of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;State Key Laboratory of Quality Control of Chinese Medicinal Materials and Decoction Pieces,Gansu Engineering Laboratory of Chinese and Tibetan Medicine Inspection and Testing Technology,Lanzhou 730000,China)

机构地区：[1]甘肃省药品检验研究院,甘肃兰州730000 [2]甘肃省中医药大学药学院,甘肃兰州730000 [3]国家中药材及饮片质量控制重点实验室,甘肃省中藏药检验检测技术工程实验室,甘肃兰州730000

出　　处：《中草药》2024年第17期5832-5840,共9页Chinese Traditional and Herbal Drugs

基　　金：国家中药材及饮片质量控制重点实验室项目(2023GSMPA-KL05)。

摘　　要：目的建立一测多评法同时测定大活络丸中16种挥发性成分[柠檬烯、桉油精、异龙脑、龙脑、苯甲酸、乙酸辛酯、5-羟甲基糠醛、丁香酚、肉桂酸、香草醛、甲基丁香酚、广藿香酮、百秋李醇、α-香附酮、(E)-藁本内酯、麝香酮]的含量。方法采用HP-5毛细管柱(30 m×0.32 mm×0.25μm);FID检测器;程序升温;进样口温度为230℃;检测器温度为250℃;分流比为20∶1;色谱柱体积流量1 mL/min。以龙脑为内参物,建立其与15种挥发性成分的相对校正因子,计算各待测组分的含量,比较外标法和一测多评法这2种方法的差异。结果一测多评法测得的结果与外标法测得的结果无显著性差异(RSD<5.0%)。结论建立大活络丸中16种挥发性成分的一测多评法,该方法简便可行,能较好地体现大活络丸中挥发性成分的整体性和差异性,可为大活络丸的质量控制提供依据。Objective To establish a quantitative analysis of multi-components by single-marker(QAMS)method for simultaneously determining the content of 16 volatile components[limonene,eucalyptol,isoborneol,borneol,benzoic acid,acetic acid octyl ester,5-hydroxymethylfurfural,eugenol,cinnamic acid,vanillin,methyleugenol,dhelwangin,patchouli alcohol,α-cyperone,(E)-ligustilide,muscone]in Dahuoluo Pills(大活络丸).Methods Using HP-5 capillary column(30 m×0.32 mm×0.25μm);FID detector;Program heating up;The inlet temperature was 230℃;The detector temperature was 250℃;The diversion ratio was 20:1;Column flow rate was 1 mL/min.Using borneol as an internal reference,establish its relative correction factors with 15 volatile components,calculate the content of each tested component,and compare the differences between the external standard method and the method of QAMS.Results There was no significant difference(RSD<5.0%)between the results obtained by the method of QAMS and the results obtained by the external standard method.Conclusion The method of QAMS was established for 16 volatile components in Dahuoluo Pills.This method is simple and feasible,and can better reflect the overall and differential characteristics of volatile components in Dahuoluo Pills.It can provide a basis for quality control of Dahuoluo Pills.

关 键 词：大活络丸 一测多评 气相色谱法 挥发性成分 柠檬烯 桉油精 异龙脑 龙脑 苯甲酸 乙酸辛酯 5-羟甲基糠醛 丁香酚 肉桂酸 香草醛 甲基丁香酚 广藿香酮 百秋李醇 Α-香附酮 (E)-藁本内酯 麝香酮 

分 类 号：R283.6[医药卫生—中药学]