基于HPLC指纹图谱和多成分定量测定结合化学模式识别的断血流质量评价  

Quality control method for Clinopodii Herba based on HPLC fingerprint and multi-component quantitative determination combined with chemical pattern recognition

作　　者：王娜 韩玲玲 张亚中 刘军玲 WANG Na;HANG Lingling;ZHANG Yazhong;LIU Junling(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Anhui Provincial Institute for Food and Drug Control,Hefei 230051,China;Key Laboratory ofQuality Research and Evaluation of Traditional Chinese Medicines of the State Food and Drug Administrat Hefei230051,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽省食品药品检验研究院,安徽合肥230051 [3]国家药监局中药质量研究与评价重点实验室,安徽合肥230051

出　　处：《中草药》2024年第17期6009-6015,共7页Chinese Traditional and Herbal Drugs

基　　金：安徽省重点研究与开发计划:十大皖药断血流产业化关键技术与标准研制(202104h04020036)。

摘　　要：目的建立断血流的HPLC指纹图谱与含量测定方法,结合化学计量学分析断血流的质量。方法采用HPLC法建立15批断血流药材的指纹图谱,通过对照品比对,指认共有峰,并测定其中4种有效成分(木犀草苷、橙皮苷、槲皮素、醉鱼草皂苷IVb)的含量,使用《中药色谱指纹图谱相似度评价系统(2012版)》、IBM SPSS Statistics 23和Simca软件进行相似度评价、聚类分析(cluster analysis,CA)、主成分分析(principal component analysis,PCA)及正交偏最小二乘判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA),对不同产地断血流的差异进行分析。结果建立的15批断血流指纹图谱共匹配出21个共有峰,通过与对照品比对,指认出木犀草苷、橙皮苷、迷迭香酸、蒙花苷、槲皮素、醉鱼草皂苷IVb 6个色谱峰,指纹图谱相似度为0.660~1.000;15批次断血流药材中木犀草苷、橙皮苷、槲皮素、醉鱼草皂苷IVb的质量分数分别为0.2029~2.7728、4.7190~16.9570、0.2520~1.0453、0.0228~0.1708mg/g;CA分析将样品分为3类;PCA提取出1个主成分;OPLS-DA分析确定木犀草苷、槲皮素2个差异标志物。结论建立的断血流HPLC指纹图谱与含量测定并结合化学计量学分析方法稳定可靠,可用于断血流药材的质量评价。Objective To establish a high-performance liquid chromatography(HPLC)fingerprint and content determination method for Duanxueliu(Clinopodii Herba),and to evaluate the quality of Clinopodii Herba through chemometric analysis.Methods HPLC was used to establish fingerprint spectra of 15 batches of Clinopodii Herba,identify common peaks by comparison with reference materials,and determine the content of four effective ingredients(luteolin-7-O-glucoside,hesperidin,quercetin,buddlejasaponins IVb).The similarity evaluation,cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least squares discriminant analysis(OPLS-DA)were performed using traditional Chinese medicine fingerprints(version 2012),IBM SPSS Statistics 23,and Simca software to analyze differences in Clinopodii Herba from different regions of origin.Results A total of 21 common peaks were matched in 15 batches of established Clinopodii Herba fingerprint spectra.Compared with the control sample,six chromatographic peaks were identified,including luteolin-7-O-glucoside,hesperidin,rosmarinic acid,buddleoside,quercetin,buddlejasaponins IVb.The similarity range of the fingerprint spectra was 0.660—1.000;The mass fractions of luteolin-7-O-glucoside,hesperidin,quercetin and buddlejasaponins IVb in 15 batches of Clinopodii Herba were 0.2029—2.7728,4.7190—16.9570,0.2520—1.0453,0.0228—0.1708 mg/g,respectively;CA divides the samples into three categories;PCA extracts one principal component;OPLS-DA was used to determine two quality difference biomarkers,luteolin-7-O-glucoside and quercetin.Conclusion HPLC fingerprint and content determination established in this study,combined with chemometric analysis methods,are stable and reliable,and can be used for quality control research of Clinopodii Herba.

关 键 词：断血流 高效液相色谱 指纹图谱 木犀草苷 橙皮苷 槲皮素 醉鱼草皂苷IVb 含量测定 化学计量学 质量评价 

分 类 号：R286.2[医药卫生—中药学]