杯叶海绵的化学成分研究  

Chemical constituents of Phyllospongia foliascens

作　　者：吴高玲 吕凤翊 范晓婷 林厚文[1] 成熙 杨帆[1,3] WU Gaolin;LYU Fengyi;FAN Xiaoting;LIN Houwen;CHENG Xi;YANG Fan(Department of Pharmacy,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China;Shanghai Tianyou Hospital,Tongji University,Shanghai 200331,China;Marine Biomedical Science and Technology Innovation Platform of Lingang Special Area,Shanghai 201306,China)

机构地区：[1]上海交通大学医学院附属仁济医院药学部,上海200127 [2]同济大学附属上海天佑医院,上海200331 [3]临港新片区海洋生物医药科技创新型平台,上海201306

出　　处：《中草药》2024年第18期6115-6122,共8页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划-青年科学家项目(2022YFC2804300);国家自然科学基金面上项目(82073758);临港新片区海洋生物医药科技创新型平台科创基金。

摘　　要：目的对采自西沙永乐群岛海域的杯叶海绵Phyllospongia foliascens进行系统的化学成分研究。方法综合运用硅胶柱色谱、十八烷基硅烷键合硅胶填料(ODS)柱色谱、Sephadex LH-20凝胶柱色谱、半制备高效液相色谱等技术进行分离纯化得到单体化合物。采用高分辨电喷雾电离质谱、核磁共振波谱、圆二色谱结合文献比对等方法鉴定其结构。对分离得到的化合物进行细胞毒活性评价。结果从杯叶海绵的石油醚萃取物中分离鉴定了7个单体化合物,包括1个新scalarane型二倍半萜:杯叶海绵萜U(1);以及已知的4个scalarane型二倍半萜:phyllofolactone Q(2)、phyllofolactone R(3)、carteriofenone I(4)、carteriofenone G(5);2个甾醇:5α,8α-epidioxycholest-6-en-3β-ol(6)和(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(7)。化合物1对Daudi细胞和Raji细胞增殖有抑制作用,半数抑制浓度(half maximal inhibitory concentration,IC50)值分别为2.23、15.24μmol/L。结论化合物1为新化合物,具有细胞毒活性,化合物4~6为杯叶海绵中首次分离得到的化合物。Objective To systematically study the chemical constituents of Phyllospongia foliascens from Yongle Island,Xisha.Methods The monomers were separated and purified by silica gel column chromatography,octadecylsilyl silica gel(ODS)column chromatography,Sephadex LH-20 gel chromatography and semi-preparative high performance liquid chromatography(HPLC).Then they were identified by high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),nuclear magnetic resonance spectroscopy(NMR)and electrostatic circular dichroism(ECD),combined with literature data comparison.The cytotoxic activity of these compounds was evaluated.Results A total of seven compounds were isolated and identified from the petroleum ether extract of P.foliascens,including one new scalarane sesterterpenoid:phyllofolactone U(1),along with four scalarane sesterterpenoids:phyllofolactone Q(2),phyllofolactone R(3),carteriofenone I(4),carteriofenone G(5)and two sterols:5α,8α-epidioxycholest-6-en-3β-ol(6),(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(7).Compound 1 exhibited the inhibitory effects on Daudi and Raji cells with half maximal inhibitory concentration(IC50)values of 2.23 and 15.24μmol·L-1,respectively.Conclusion Compound 1 is a new compound with cytotoxic activity,and compounds 4—6 was isolated from P.foliascens for the first time.

关 键 词：Phyllospongia foliascens 海绵 scalarane型二倍半萜 海洋天然产物 抗肿瘤活性 phyllofolactone U 

分 类 号：R284.1[医药卫生—中药学]