大疱性类天疱疮患者疱液中HBD-2、CXCR5及ECP的表达水平及意义  

Expression and significance of HBD-2,CXCR5,and ECP in the blister fluid of patients with bullous pemphigoid

作　　者：李丙南 刘桂丽 曹娜 张海祥 赵子申 LI Bing-nan;LIU Gui-li;CAO Na;ZHANG Hai-xiang;ZHAO Zi-shen(Department of Dermatology,Cangzhou People's Hospital,Cangzhou 061000,Hebei,China;Department of Nursing,Cangzhou Medical College,Cangzhou 061000,Hebei,China)

机构地区：[1]沧州市人民医院皮肤科,河北沧州061000 [2]沧州医学高等专科学校护理系,河北沧州061000

出　　处：《川北医学院学报》2024年第10期1338-1342,共5页Journal of North Sichuan Medical College

基　　金：河北省医学科学研究项目(20211626)。

摘　　要：目的:探究大疱性类天疱疮(BP)患者疱液中人类β防御素2(HBD-2)、趋化因子受体5(CXCR5)、嗜酸性粒细胞阳离子蛋白(ECP)的表达水平及意义。方法:选取80例BP患者为BP组;50例非BP患者为非BP组,根据BP活动性皮损面积(A)和活动性皮损数目(B)将BP组分为重度组、中度组及轻度组;随访6个月,根据疾病复发及并发症发生情况将BP组患者分为预后不良组及预后较好组,采用ELISA法检测BP患者及非BP患者、不同病情程度BP患者及不同预后结果BP患者之间HBD-2、CXCR5、ECP水平差异,并分析HBD-2、CXCR5、ECP水平与BP严重程度及患者预后的相关性。结果:BP组HBD-2、CXCR5、ECP水平均高于非BP组(P<0.05)。不同病情程度BP患者疱液HBD-2、CXCR5、ECP水平比较:重度组>中度组>轻度组(P<0.05)。相关性分析显示,BP患者疱液HBD-2、CXCR5、ECP水平与病情严重程度均呈正相关关系(P<0.05)。疱液HBD-2、CXCR5、ECP水平联合检测评估BP严重程度的曲线下面积(AUC)大于各指标单独检测(P<0.05)。预后不良组HBD-2、CXCR5、ECP水平均高于预后较好组(P<0.05)。疱液HBD-2、CXCR5、ECP水平联合检测预测BP患者预后的AUC大于各指标单独检测(P<0.05)。结论:大疱性类天疱疮患者疱液中HBD-2、CXCR5、ECP水平异常升高与疾病严重程度相关,且各指标联合检测对BP严重程度及患者预后具有预测价值。Objective:To investigate the expression and significance of humanβ-defensin 2(HBD-2),chemokine receptor 5(CXCR5),and eosinophil cationic protein(ECP)in the blister fluid of patients with bullous pemphigoid(BP).Methods:80 BP patients and 50 non BP patients were selected as the BP group and the non BP group.The BP group was divided into severe group,moderate group,and mild group according to the area of active skin lesions(A)and the number of active skin lesions(B).During a 6-month follow-up,patients in the BP group were divided into a poor prognosis group and a better prognosis group based on the occurrence of disease recurrence and complications.The levels of HBD-2,CXCR5,and ECP in BP patients and non BP patients,BP patients with different degrees of illness,and BP patients with different prognosis results were detected by ELISA.The correlation between the levels of HBD-2,CXCR5,and ECP in blister fluid and the severity of BP and patient prognosis was analyzed.Results:The levels of HBD-2,CXCR5,and ECP in the BP group were higher than those in the non-BP group(P<0.05).The levels of HBD-2,CXCR5,and ECP in the severe group were higher than those in the moderate and mild groups,and the levels in the moderate group were higher than those in the mild group(P<0.05).Correlation analysis showed that the levels of HBD-2,CXCR5,and ECP in blister fluid were positively correlated with the severity of BP(P<0.05).The combined detection of HBD-2,CXCR5,and ECP levels in blister fluid had a higher AUC value in assessing BP severity compared to each indicator alone(P<0.05).The levels of HBD-2,CXCR5,and ECP in the poor prognosis group were higher than those in the better prognosis group(P<0.05).The combined detection of HBD-2,CXCR5,and ECP levels in blister fluid had a higher AUC value in predicting the prognosis of BP patients compared to each indicator alone(P<0.05).Conclusion:The abnormal increase in HBD-2,CXCR5,and ECP levels in the blister fluid of BP patients is related to the severity of the disease,and the combined detection

关 键 词：大疱性类天疱疮 疱液 人类β防御素2 趋化因子受体5 嗜酸性粒细胞阳离子蛋白 

分 类 号：R753.1[医药卫生—皮肤病学与性病学]