病原菌侵染下细胞外ATP对光系统Ⅱ光化学反应的调节作用  

Regulation of the photochemical reaction of photosystemⅡby extracellular ATP under pathogen infection

作　　者：曹璐璐 金昱杉 张悦婧 王涵琦 邓雅欣 孙坤[1] 张继[1,2] 贾凌云[1,2] 冯汉青[1,2] CAO Lu-lu;JIN Yu-shan;ZHANG Yue-jing;WANG Han-qi;DENG Ya-xin;SUN Kun;ZHANG Ji;JIA Ling-yun;FENG Han-qing(College of Life Science,Northwest Normal University,Lanzhou 730070,China;Key Laboratory of Biological Resources Development and Biological Products in the Northwest Plateau,China General Chamber of Commerce,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]西北师范大学中国商业联合会西北高原生物资源开发与生物制品重点实验室,兰州730070

出　　处：《兰州大学学报（自然科学版）》2024年第5期593-598,共6页Journal of Lanzhou University(Natural Sciences)

基　　金：国家自然科学基金项目(32460074,31870246);甘肃省自然科学基金重点项目(24JRRA124);甘肃省重点研发计划项目(22YF7NA119,23YFNA0022);甘肃省高等学校产业支撑计划项目(2023CYZC-20);甘肃省科技重大专项(22ZD1NA001);西北师范大学新农村发展研究院开放基金;西北师范大学研究生科研资助项目(2021KYZZ01037);西北师范大学优秀博士论文培养资助项目;甘肃省科技计划资助(优秀博士)项目(23JRRA697,23JRRA707)。

摘　　要：以野生型Col-0和细胞外ATP(e ATP)受体突变型dorn1-3拟南芥为实验材料,探究e ATP在两种丁香假单胞菌(毒性Pst DC3000和非毒性Pstavr B)侵染下的水平变化以及对其光化学反应的调节作用.结果表明,Pst DC3000和Pstavr B侵染均导致拟南芥叶片中e ATP水平、光适应下叶片的最大光化学量子产率(F_(v)′/F_(m)′)、光适应下PSⅡ实际光化学效率(Y(Ⅱ))、光合电子传递速率(ETR)降低而非调节性能量耗散的量子产额Y(NO)升高.Pst DC3000侵染下,dorn1-3拟南芥植株F_(v)′/F_(m)′、Y(Ⅱ)、ETR略低于野生型,Y(NO)略高于野生型,但均未达显著性差异;在Pstavr B侵染下,dorn1-3拟南芥植株F_(v)′/F_(m)′、Y(Ⅱ)、ETR显著低于野生型植株,Y(NO)显著高于野生型.在病原菌侵染下,e ATP受体的突变会增强病原菌对PSⅡ的抑制作用,且在非毒性病原菌侵染下该效应更为明显.在非毒性病原菌侵染对植物光化学反应影响的过程中e ATP具有重要的调控作用.In order to explore the changes in extracellular ATP(eATP)levels and regulation of photo-chemical reaction by eATP under pathogen infection,a wild type Col-0 and eATP receptor mutant dorn1-3 of Arabidopsis were used as experimental materials,and it was found that the infection with either virulent pathogen(PstDC3000)or avirulent pathogen(PstavrB)resulted in a decrease of eATP levels,values of the maximum photochemical efficiency under light adaptation(F_(v)′/F_(m)′),actual photo-chemical efficiency of PSⅡ(Y(Ⅱ))and photosynthetic electronic transmission rate(ETR),but an increase in the quantum yield of non-regulated energy dissipation(Y(NO))of the leaves.By a com-parison between wild type Col-0 and eATP receptor mutant dorn1-3 of Arabidopsis seedlings,it was revealed that,under PstDC3000 infection,the values of F_(v)′/F_(m)′,Y(Ⅱ)and ETR of dorn1-3 plants were slightly lower than those of the wild type,while the Y(NO)was slightly higher than those of the wild type.Under PstavrB infection,the values of F_(v)′/F_(m)′,Y(Ⅱ)and ETR of the dorn1-3 plants were signifi-cantly lower than those of wild type plants,while Y(NO)was significantly higher.The results above indicated that the mutation of eATP receptor could exacerbate the inhibition of the pathogen infection on the photochemical reaction of PSⅡunder pathogen infection,and such effect of the mutation of eATP receptor was more significant under PstavrB infection.It was suggested that eATP could play an impor-tant role in regulating the photochemical reaction of PSⅡunder pathogen infection.

关 键 词：拟南芥 丁香假单胞菌 胞外ATP 叶绿素荧光参数 

分 类 号：Q945[生物学—植物学]