Sysmex XN-9000体液模式分类脑脊液有核细胞的准确性评估  

Accuracy evaluation of Sysmex XN-9000 body fluid mode classification for cerebrospinal fluid nucleated cells

作　　者：马玲辉 黄坚 陈雅斌[1] 陈慧丹[3] 温小梅[3] Ma Linghui;Huang Jian;Chen Yabin;Chen Huidan;Wen Xiaomei(Department of Clinical Laboratory,Quanzhou First Hospital Affiliated to Fujian Medical University,Quanzhou 362000,China;Department of Clinical Laboratory,900TH Hospital of Joint Logistics Support Force,Fuzhou 350000,China;Department of Clinical Laboratory,Fujian Provincial Hospital,Fuzhou 350001,China)

机构地区：[1]福建医科大学附属泉州第一医院检验科,泉州362000 [2]联勤保障部队第九〇〇医院检验科,福州350000 [3]福建省立医院检验科,福州350001

出　　处：《实用医技杂志》2024年第8期559-562,I0001,I0002,共6页Journal of Practical Medical Techniques

摘　　要：目的评估Sysmex XN-9000体液模式分类脑脊液有核细胞的准确性。方法选取2022年8月至2023年5月福建省立医院检验科的356例脑脊液标本同时进行Sysmex XN-9000体液模式检测和人工镜检,记录各有核细胞百分比。采用Passing and Bablok回归和Bland-Altman图评估仪器法和人工镜检结果的一致性。结果脑脊液有核细胞数(0~15)×10^(6)/μl组淋巴细胞百分比(LY-BF%)、高荧光强度有核细胞百分比(HF-BF%)仪器法和人工镜检结果一致性较低(线性方程Y=21.640+0.800X,Y=0.000+0.250X)。有核细胞数(16~30)×10^(6)/μl组中性粒细胞百分比(NE-BF%)、LY-BF%、单核细胞百分比(MO-BF%)三者一致性高(线性方程Y=-1.016+1.000X,Y=2.933+0.967X,Y=0.595+1.038X);有核细胞数>30×10^(6)/μl组NE-BF%、LY-BF%、MO-BF%三者一致性高(线性方程Y=-0.433+1.000X,Y=2.296+0.954X,Y=1.783+0.943X)。结论有核细胞数(0~15)×10^(6)/μl的脑脊液标本,应视临床需求不分类或全部人工镜检。>15×10^(6)/μl的脑脊液标本,仪器检测EO-BF%、HF-BF%比例极低时,可按照仪器检测结果报告。Objective To evaluate the accuracy of Sysmex XN-9000 body fluid mode classification for cerebrospinal fluid(CSF)nucleated cells.Methods Three hundred and fifty-six CSF samples from the clinical laboratory of Fujian Provincial Hospital from August 2022 to May 2023 were selected for both Sysmex XN-9000 body fluid mode detection and manual microscopy,and the percentage of nucleated cells was recorded.Passing and Bablock regression and Bland Altman plot were used to evaluate the consistency of instrument and manual microscopy results.Results The consistency between the instrument and manual microscopy results of the LY-BF%and HF-BF%in the group with nucleated cells in CSF(0~15)×10^(6)/μl was low(linear equation Y=21.640+0.800X,Y=0.000+0.250X).The consistency of the NE-BF%,LY-BF%,and MO-BF%in the group with nucleated cells(16~30)×10^(6)/μl was high(linear equation Y=-1.016+1.000X,Y=2.933+0.967X,Y=0.595+1.038X).The consistency of the NE-BF%,LY-BF%,and MO-BF%in the group with nucleated cells>30×10^(6)/μl was high(linear equation Y=-0.433+1.000X,Y=2.296+0.954X,Y=1.783+0.943X).Conclusion CSF with a nuclear cell count of(0~15)×10^(6)/μl should not be classified or undergone manual microscopy depending on clinical needs.When the instrument detects a very low proportion of EO-BF%and HF-BF%in a CSF sample with nucleated cell count higher than 15×10^(6)/μl,it can be directly reported,based on the instrument results.

关 键 词：诊断 脑脊液 有核细胞分类 准确性评估 

分 类 号：R446.14[医药卫生—诊断学]