基于GBS测序的3个野生单环刺螠群体遗传多样性和群体结构分析  

Analysis of Genetic Diversity and Population Structure of Three Wild Urechis unicinctus Populations Based on GBS Sequencing

作　　者：尤宏争[1] 孟睦涵 马林[1] 郑艳坤 王永辉 孙阳 毕相东 孙学亮 YOU Hongzheng;MENG Muhan;MA Lin;ZHENG Yankun;WANG Yonghui;SUN Yang;BI Xiangdong;SUN Xueliang(Tianjin Fisheries Research Institute,Tianjin 300221,China;Tianjin Agricultural Development Service Center,Tianjin 300211,China;Tangshan Ruihui Aquaculture Co.,Ltd.,Tangshan 063500,Hebei,China;Dalian Marine Development Service Center,Dalian 116023,Liaoning,China;College of Fisheries,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津市水产研究所,天津300221 [2]天津市农业发展服务中心,天津300211 [3]唐山瑞辉水产养殖有限公司,河北唐山063500 [4]大连市海洋发展事务服务中心,辽宁大连116023 [5]天津农学院水产学院,天津300384

出　　处：《四川农业大学学报》2024年第5期1145-1151,共7页Journal of Sichuan Agricultural University

基　　金：天津市科技计划项目(24ZYCGSN00110);天津市科技计划项目(23ZYCGSN00780);天津市科技计划项目(22ZYCGSN00050);大连市科技创新基金项目(2018J12SN082)。

摘　　要：【目的】旨在评估我国野生单环刺螠群体的遗传多样性和群体结构。【方法】采集大连、唐山、烟台3个群体样本,利用GBS测序技术获取SNP标记位点信息,分别对其期望杂合度、观测杂合度、核苷酸多样性、分子方差、聚类及群体结构等进行分析。【结果】3个野生单环刺螠群体观测杂合度为0.212~0.223,期望杂合度为0.239~0.257;核苷酸多样性在0.252~0.271之间。遗传变异主要来源于单环刺螠群体内部。3个自然群体可分成2个遗传群体,样本未根据地理位置表现出明显的群体结构。【结论】我国野生单环刺螠具有中等遗传多样性。【Objective】The purpose of this study was to evaluate the genetic diversity and population structure of wild Urechis uniconctus populations in China.【Method】Three population samples were col⁃lected from Dalian,Tangshan,and Yantai,and SNP marker site information was obtained by using GBS sequencing technology.The expected heterozygosity,observed heterozygosity,nucleotide diversity,mo⁃lecular variance,clustering,and population structure were analyzed.【Result】The observed heterozy⁃gosity of three wild populations of Urechis uniconctus was 0.212-0.223.The expected heterozygosity was 0.239-0.257.The diversity of nucleotides ranged from 0.252 to 0.271.Genetic variation was mainly de⁃rived from the population of Urechis uniconctus.Three wild populations can be divided into two genetic populations,but the samples did not show a clear population structure based on geographical location.【Conclusion】The wild Urechis uniconctus in China has middle genetic diversity.

关 键 词：单环刺螠 GBS测序 野生群体 遗传多样性 群体结构 

分 类 号：S931.5[农业科学—渔业资源]