中枢神经系统感染患者脑脊液中12种细胞因子的变化及临床意义  

Changes in 12 cytokines in the cerebrospinal fluid of patients with CNS infections and their clinical significance

作　　者：张玥 郑璐 陈园园 裴蕴锋 于魁魁 顾锋 陈永强 ZHANG Yue;ZHENG Lu;CHEN Yuanyuan(Department of Clinical Laboratory,Xuzhou Central Hospital,Xuzhou 221000,China;不详)

机构地区：[1]徐州市中心医院检验科,江苏徐州221000 [2]徐州市中心医院神经内科,江苏徐州221000 [3]徐州医科大学徐州临床学院,江苏徐州221000

出　　处：《中风与神经疾病杂志》2024年第10期923-927,共5页Journal of Apoplexy and Nervous Diseases

基　　金：江苏省卫生健康委科研项目(M2021025);徐州市卫健委“彭城英才—医学青年后备人才”项目(XWRCHT20220006)。

摘　　要：目的探讨12种细胞因子(IFN-α、IFN-γ、TNF-α、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IL-12p70和IL-17)在中枢神经系统感染性疾病患者脑脊液中的变化特征及其临床意义。方法收集徐州市中心医院临床诊断为中枢神经系统感染患者52例,对采集的脑脊液进行脑脊液常规、生化以及脑脊液细胞学检测;利用流式细胞仪检测脑脊液中12种细胞因子的变化情况。结果在检测的12种细胞因子中仅IL-5、IL-6和IL-83个细胞因子的浓度显著升高,阳性率分别为23.08%、42.31%和96.15%;与脑脊液中白细胞计数和中性粒细胞百分比呈显著正相关,与淋巴细胞的百分比呈负相关。另外,与白细胞计数结果相一致,细胞因子IL-5、IL-6和IL-8在患者治疗获益后均明显下降。结论脑脊液中IL-5、IL-6和IL-8水平的检测可作为今后中枢神经系统感染患者诊治以及病情严重程度判断的依据。Objective To examine the pattern of changes in 12 cytokines(IFN-α,IFN-γ,TNF-α,IL-1β,IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12p70,and IL-17)in the cerebrospinal fluid(CSF)of patients with central nervous system(CNS)infections and their clinical significance.Methods Fifty-two patients clinically diagnosed with CNS infections at our hospital were enrolled in the study.CSF was collected for CSF analysis,biochemical analysis,and cytology.Changes in the CSF levels of 12 cytokines were measured by flow cytometry.Results Only IL-5,IL-6,and IL-8 were significantly increased in the CSF,and the corresponding positive rates were 23.08%,42.31%,and 96.15%,respectively.High levels of IL-5,IL-6,and IL-8 were positively correlated with white blood cell(WBC)count and neutrophil percentage but negatively correlated with lymphocyte percentage in the CSF.Consistent with the WBC count,IL-5,IL-6,and IL-8 levels in the CSF were significantly decreased after effective treatment.Conclusion The levels of IL-5,IL-6,and IL-8 in CSF can be used as potential markers for the diagnosis,treatment,and severity assessment of CNS infections.

关 键 词：脑脊液 细胞因子 中枢神经系统感染 多重微球流式免疫荧光法 

分 类 号：R446.62[医药卫生—诊断学]