The pentatricopeptide repeat protein TCD6 functions RNA editing and cleavage of ndhA and is required for chloroplast development in early rice seedlings  

作　　者：Yunguang Sun Licheng Kuang Jinglin Wang Mengshuang Gu Yu Chen Xiaobiao Pan Dongzhi Lin Yanjun Dong 

机构地区：[1]College of Life Sciences,Shanghai Normal University,Shanghai,200234,China [2]Crop Institute,Taizhou Academy of Agricultural Sciences,Zhejiang,Linhai,317000,China [3]Shanghai Key Laboratory of Plant Molecular Sciences,Shanghai,200234,China [4]Institute of Genetics,Shanghai Normal University,Shanghai,200234,China

出　　处：《Crop Design》2024年第3期4-13,共10页作物设计（英文）

基　　金：funded by Minister of Science and Technology of China(MOST)(2016YFD0100902);the Shanghai Municipal Science and Technology Commission(19391900200);Agriculture Research System of Shanghai,China(Grant No.202003 and 2021003);the Natural Sci-ence Foundation of China(No.30971552).

摘　　要：Pentatricopeptide repeat(PPR)proteins compose one of the largest protein families in higher plants and play a role in regulating organellar gene expression.In this study,we discovered that a new rice mutant tcd6 exhibited albino phenotype and aberrant chloroplast before the three-leaf(autotrophic)seedling stage.Through Map-based cloning and complementation tests,it was shown that TCD6 encodes a chloroplast-located PPR protein,with 14 PPR motifs and an atypical DYW-like motif.In addition,the disruption of TCD6 hindered the nuclear-encoded polymerase(NEP)-dependent transcript levels for plastid genes and led to defects in the cleavage and editing of ndhA(encoding NDH subunit)in early tcd6 mutant seedlings.Taken together,our results indicate that TCD6 is indispensable for chloroplast development and involves in RNA editing and cleavage of ndhA during early seedling(autotrophic)growth of rice.

关 键 词：Rice(Oryza sativa L.) PPR protein Chloroplast development RNA editing 

分 类 号：S511[农业科学—作物学]