In vitro and in silico approach for the evaluation of enzyme inhibitory potential of Kadipatta (Murraya koenigii) collected from western Nepal  

作　　者：Suman Prakash Pradhan Ishan Subedi Kapil Adhikari Ashok GC Sujan Prakash Pradhan Mukti Ram Aryal Gopal Prasad Ghimire Bishnu Prasad Pandey 

机构地区：[1]Department of Chemical Science and Engineering,Kathmandu University,PO Box:6250 Kavre,Dhulikhel,Bagmati Province 45210,Nepal [2]Aquatic Ecology Centre,Kathmandu University,Dhulikhel 45210,Nepal [3]Department of Environmental Science and Engineering,Kathmandu University,Dhulikhel 45210,Nepal [4]Department of Botany,Tri-Chandra Multiple Campus,Kathmandu 44600,Nepal [5]Department of Applied Sciences,Western Regional Campus,Pokhara 33700,Nepal

出　　处：《Clinical Traditional Medicine and Pharmacology》2024年第4期44-53,共10页临床传统医学和药理学（英文）

基　　金：supported by a UGC collaborative research grant(Award No:CRG-78/79-S&T-01)and also supported by H-Plant Nepal Private Limited.

摘　　要：Background:Murraya koenigii(L.)Spreng.(Rutaceae)is an evergreen shrub distributed over the sub-tropical to lower temperate climatic zone of Nepal which has been used as spice in culinary practice and traditionally being used to treat bites of poisonous animals,dysentery,skin diseases,vomiting,diarrhea,and inflammations.Objective:The main objective of this study is to study the enzyme inhibition potential of different extracts of M.koenigii leaves collected from western Nepal.Methods:Total flavonoid content,total phenolic content,in vitro antioxidant,𝛼α-amylase,lipase,elastase,tyrosinase,and cholinesterase inhibition activities analysis of M.koenigii in ethyl acetate,methanol,and water extract were evaluated.In addition,high-resolution mass spectrometry-based profiling of flavonoid molecules and their docking analysis were performed.Results:Results revealed the noteworthy radical scavenging activity of ethyl acetate extract against ABTS and DPPH.Among the analyzed enzymes,M.koenigii extracts revealed the highest inhibitory potential towards acetylcholinesterase(AChE)and butyrylcholinesterase(BChE)and only water extract showed the inhibition of tyrosinase.The HPLC-ESI-QTOF-MS analysis revealed the presence of Rutin,Quercetin,Ferulic acid,Hyperoside,Kaempferol-7-O-glucoside,and Morin in M.koenigii.Further,in silico docking analysis revealed the significant binding energy of these flavonoid molecules;especially Rutin with BChE.Conclusion:The ethyl acetate and methanolic extracts of M.koenigii showed paramount flavonoid and phenolic content along with noteworthy potential towardsthe inhibition of neuro-degradative enzymes such as BChE which can be characterized further for pharmacological applications.Further study on the isolation of metabolites and their pharmacological properties from M.koenigii is recommended.

关 键 词：Murraya koenigii 𝛼α-amylase LIPASE ELASTASE TYROSINASE Cholinesrerase 

分 类 号：R28[医药卫生—中药学]