高州油茶与小果油茶种仁蛋白质组差异比较  

Proteome differences between Camellia drupifera and Camellia meiocarpa seed kernels

作　　者：魏尚霖 李晨蕾 廖柏勇 程俊森 代文魁 姜维 王溢 李永泉 WEI Shanglin;LI Chenlei;LIAO Boyong;CHENG Junsen;DAI Wenkui;JIANG Wei;WANG Yi;LI Yongquan(College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,Guangdong,China;Scarce and Quality Economic Forest Engineering Technology Research Center of Common Colleges and Universities in Guangdong Province,Guangzhou 510225,Guangdong,China)

机构地区：[1]仲恺农业工程学院园艺园林学院,广东广州510225 [2]广东省普通高校优稀特色经济林果工程技术研究中心,广东广州510225

出　　处：《经济林研究》2024年第3期10-24,共15页Non-wood Forest Research

基　　金：广东省重点领域研发计划项目(2020B020215003);广东省林业科技创新项目(2024KJCX004,2023KJCX006);广东省普通高校优稀特色经济林果工程技术研究中心项目(2022GCZX002);仲恺农业工程学院研究生教育创新计划项目(KA23YY40514)。

摘　　要：【目的】探索高州油茶Camellia drupifera和小果油茶Camellia meiocarpa调控种仁油脂合成、积累和代谢的关键差异蛋白质。【方法】使用非标记技术对2种油茶种仁的蛋白质组进行鉴定,采用亚细胞定位分析、结构域分析、聚类分析、GO和KEGG分析、蛋白质互作网络等生物信息学分析方法进行差异表达蛋白质的筛选和功能分类。【结果】在油茶种仁中共鉴定出5712个蛋白质,其中740个蛋白质表达量差异显著。与小果油茶相比,337个差异蛋白在高州油茶种仁中上调表达,403个差异蛋白在高州油茶种仁中下调表达。GO功能分类结果表明,差异表达蛋白质主要参与氧化还原的过程以及核糖体的组成。KEGG富集分析结果显示,视黄醇代谢、丙酮酸代谢、萘降解、氧化磷酸化和核糖体是差异表达蛋白质显著富集的关键代谢通路。与小果油茶相比,高州油茶种仁的6个显著差异表达蛋白质(淀粉合酶、ADP-葡萄糖焦磷酸化酶、海藻糖-6-磷酸合酶/磷酸酶、UDP-葡萄糖焦磷酸化酶、果糖激酶和己糖激酶)在淀粉和蔗糖代谢通路下调,可能与脂肪酸的合成和积累有关。此外,在高州油茶种仁中,11个显著差异表达蛋白质参与了与脂肪酸代谢相关的4个重要通路,其中3个显著差异表达蛋白质参与多个通路,长链酰基辅酶A合成酶和烯酰基载体蛋白还原酶/反式-2-烯酰-辅酶A还原酶在脂肪酸的生物合成、脂肪酸降解和脂肪酸延伸通路中下调,而酰基-[酰基载体蛋白]去饱和酶在脂肪酸的生物合成通路中下调,在不饱和脂肪酸的生物合成通路中上调。【结论】与糖类和脂肪酸代谢相关的蛋白在高州油茶种仁中显著差异表达,揭示了高州油茶和小果油茶种仁中的糖类和脂肪积累与代谢模式的差异,推测糖类合成相关蛋白表达下调与糖类在油茶种仁中转化为脂肪酸有关。差异表达蛋白质在不饱和脂肪酸的生物【Objective】Exploring key differential proteins in Camellia drupifera and Camellia meiocarpa regulating oil synthesis,accumulation and metabolism in seed kernels.【Method】The proteomics of the two seed kernels were identified using label-free technology,the screening and functional classification of differentially expressed proteins were performed by bioinformatics techniques such as subcellular localisation analysis,structural domain analysis,volcano diagram,clustering heatmap,GO and KEGG analysis,and protein-protein interaction networks.【Result】A total of 5712 proteins were identified in two kinds of seed kernels,and the expression of 740 proteins was significantly different,of which 337 were up-regulated and 403 were down-regulated in C.drupifera seed kernels.GO functional classification results indicated that the differentially expressed proteins were mainly involved in oxidation-reduction process as well as the composition of ribosomes.KEGG enrichment analysis revealed that retinol metabolism,pyruvate metabolism,naphthalene degradation,oxidative phosphorylation,and ribosome were the key metabolic pathways significantly enriched in differentially expressed proteins.Six significantly differentially expressed proteins,starch synthase,glucose-1-phosphate adenylyltransferase,6-phosphotrihalose trihalose synthase/phosphatase,UTP-glucose-1-phosphate uridylyltransferase,fructokinase and hexokinase were down-regulated in starch and sucrose metabolic pathway in the seed kernels of C.drupifera compared to C.meiocarpa,which may be related to fatty acid synthesis and accumulation.In addition,11 significantly differentially expressed proteins were found involved in 4 important pathways related to fatty acid metabolism in the seed kernels of C.drupifera,3 of which were involved in more than one pathway,long-chain acyl-CoA synthetase and mitochondrial enoyl-[acyl-carrier protein]reductase/trans-2-enoyl-CoA reductase were down-regulated in fatty acid biosynthesis,fatty acid degradation and fatty acid elongation pat

关 键 词：高州油茶 小果油茶 种仁 蛋白质组 非标记技术 

分 类 号：S601[农业科学—园艺学] S794.4