不同强度UVA对银杏杂交子代生理生化的影响  

The effect of different intensities of UVA on the physiology and biochemistry of ginkgo hybrid offspring

作　　者：赵群[1,2,3] 王改萍[1,2,3] 徐意 赵慧琴 王峥 翟金庭 ZHAO Qun;WANG Gaiping;XU Yi;ZHAO Huiqin;WANG Zheng;ZHAI Jinting(Forestry and Grass College,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Soil and Water Conservation College,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Yancheng Forest Farm,Yancheng 224057,Jiangsu,China)

机构地区：[1]南京林业大学林草学院,江苏南京210037 [2]南京林业大学水土保持学院,江苏南京210037 [3]南京林业大学南方现代林业协同创新中心,江苏南京210037 [4]盐城林场,江苏盐城224057

出　　处：《经济林研究》2024年第3期58-68,共11页Non-wood Forest Research

基　　金：江苏省科技计划(资金)项目(BE2021367)。

摘　　要：【目的】探究不同强度UVA对银杏杂交子代可溶性糖、可溶性蛋白、抗氧化酶活性、黄酮代谢相关酶活性以及总黄酮含量的影响,得出提高银杏总黄酮含量的最佳光质处理,为银杏的栽培与选育提供理论依据。【方法】以二年生银杏杂交子代实生苗为试验材料,设置不同强度的紫外光UVA(395~405 nm)处理,在相同强度的白光(300μmol·m^(2)·s^(-1))下分别添加2、4、6根UVA紫外灯,紫外光强为20μmol·m^(-2)·s^(-1)(UVA-2)、40μmol·m^(-2)·s^(-1)(UVA-4)、60μmol·m^(-2)·s^(-1)(UVA-6),以白光(300μmol·m^(2)·s^(-1))做为对照(CK)。【结果】不同强度UVA处理对银杏杂交子代各项生理指标都存在显著影响,随着UVA强度的增加,银杏杂交子代可溶性糖和可溶性蛋白含量呈现逐渐上升的趋势。金叶银杏SOD、POD、PAL、C4H、4CL酶活性随着UVA强度的增加呈现先增加后减小的趋势,绿叶银杏SOD酶活性随着UVA强度的增加而增加,而POD、PAL、C4H、4CL酶活性呈现先增后减的趋势。银杏杂交子代总黄酮含量、DPPH和ABTS自由基清除率呈现先增后减的趋势,在UVA-4处理20 d时达到最大,绿叶银杏总黄酮含量最高,为28.65 mg·g^(-1),比CK处理高56.3%。除了SOD和POD外,金叶银杏其他生理指标都低于绿叶银杏。相关性结果表明,银杏各生理指标间都呈现极显著正相关。多因素方差分析发现叶色、处理时间以及UVA强度对银杏各项生理指标都存在极显著影响,并且UVA强度和处理时间两者的交互效应对抗氧化酶活性、黄酮代谢相关酶活性、黄酮含量以及DPPH、ABTS自由基清除率存在极显著影响。【结论】不同强度UVA处理对银杏杂交子代各指标存都在显著影响,40μmol·m^(-2)·s^(-1)的UVA处理20 d绿叶银杏总黄酮含量最高。【Objective】To explore the effects of different intensities of UVA on soluble sugars,soluble proteins,antioxidant enzyme activity,flavonoid metabolism-related enzyme activity,and total flavonoid content in ginkgo hybrid offspring,and to obtain the optimal light quality treatment to improve the total flavonoid content of Ginkgo biloba,providing a theoretical basis for the cultivation and breeding of ginkgo hybrid offspring.【Method】Using two-year ginkgo hybrid offspring seedlings as experimental materials,different intensities of ultraviolet light UVA(395-405 nm)treatment were set up.Under the same intensity of white light(300μmol·m^(2)·s^(-1)),2,4,and 6 UVA ultraviolet lamps were added,with a UV intensity of 20μmol·m^(-2)·s^(-1)(UVA-2),40μmol·m^(-2)·s^(-1)(UVA-4),60μmol·m^(-2)·s^(-1)(UVA-6).White light(300μmol·m^(2)·s^(-1))was used as the control(CK).【Result】Different intensities of UVA treatment had a significant impact on various physiological indicators of ginkgo hybrid offspring.With the increase of UVA intensity,the content of soluble sugars and soluble proteins showed a gradually increasing trend.The SOD,POD,PAL,C4H,and 4CL enzyme activities of golden-leaved G.biloba showed a trend of first increasing and then decreasing with the increase of UVA intensity.The SOD enzyme activity of green-leaved G.biloba increased with the increase of UVA intensity,while the POD,PAL,C4H,and 4CL enzyme activities showed a trend of first increasing and then decreasing.The total flavonoid content,DPPH,and ABTS free radical scavenging rate of ginkgo hybrid offspring showed a trend of first increasing and then decreasing,reaching their maximum after 20 days of UVA-4 treatment.The total flavonoid content of green-leaved G.biloba was the highest,at 28.65 mg·g^(-1),which was 56.3%higher than that of CK treatment.Except for SOD and POD,other physiological indicators of golden-leaved G.biloba were lower than those of green-leaved G.biloba.The correlation results showed that there were highly significant posit

关 键 词：银杏杂交子代 UVA 黄酮 

分 类 号：S687.1[农业科学—观赏园艺]