Establishment of a Lateral Flow Dipstick Detection Method for Influenza A Virus Based on CRISPR/Cas12a System  

作　　者：Xiaoyan Zhao Ximing Zheng Xiyong Yang Qi Guo Yi Zhang Jun Lou 

机构地区：[1]Department of Clinical Laboratory,Zhumadian Central Hospital,Zhumadian City,Henan Province,China [2]Laboratory of Virology,Beijing Key Laboratory of Etiology of Viral Diseases in Children,Capital Institute of Pediatrics,Beijing,China [3]National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,China

出　　处：《China CDC weekly》2024年第37期946-952,I0008,共8页中国疾病预防控制中心周报（英文）

摘　　要：Objective:This study aimed to develop a rapid,visual PCR-CRISPR/Cas12-LFD method for detecting influenza A by utilizing the conserved region of the matrix protein gene.Method:We crafted universal degradation primers and clustered regularly interspaced short palindromic repeats RNA(CRISPR RNA,crRNA)targeting the conserved matrix protein gene of the influenza virus(IFV),integrated with lateral flow dipstick(LFD)technology.This new PCR-CRISPR/Cas12-LFD approach was designed to determine its sensitivity and specificity through the analysis of various clinical samples collected in 2023.Results:The developed nucleic acid assay for influenza A viruses(IAV)demonstrated a sensitivity of 10 copies/μL without cross-reactivity with other respiratory pathogens.Evaluation of 82 clinical samples showed high concordance with results from fluorescent Polymerase Chain Reaction(PCR),achieving a kappa value of 0.95.Conclusion:A highly sensitive and specific PCRCRISPR/Cas12-LFD method has been successfully established for the detection of influenza A,offering a robust tool for its diagnosis and aiding in the prevention and control of this virus.

关 键 词：PREVENTION DIAGNOSIS SPECIFICITY 

分 类 号：R511.7[医药卫生—内科学]