基质辅助激光解吸电离飞行时间质谱在流感嗜血杆菌鉴定中的应用  

Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in identification of Haemophilus influenzae

作　　者：张雪英 叶慧 Zhang Xueying;Ye Hui(Nanjing Adikang Medical Laboratory Co.,Ltd.,Nanjing 211112,Jiangsu,China)

机构地区：[1]南京艾迪康医学检验所有限公司,江苏南京211112

出　　处：《实用检验医师杂志》2024年第3期270-273,共4页Chinese Journal of Clinical Pathologist

摘　　要：目的分析基质辅助激光解吸电离飞行时间质谱(MALDI-TOP MS)在流感嗜血杆菌鉴定中的应用。方法收集2022年1—12月南京艾迪康医学检验所有限公司实验室接收的1300份呼吸道感染患者的痰液或咽拭子样本培养和分离的嗜血杆菌,经16SrDNA序列测定检出50株为流感嗜血杆菌;分别使用MALDI-TOP MS检测系统、Analytic Products INC(API)生化细菌鉴定系统以及纸片法卫星试验对流感嗜血杆菌进行鉴定,比较并分析3种方法对流感嗜血杆菌的检出率、检测时间、检测成本及读取方式。结果MALDI-TOP MS检测系统对流感嗜血杆菌的检出率为98.0%,API生化细菌鉴定系统的检出率为90.0%,纸片法卫星试验的检出率为74.0%。MALDI-TOP MS对流感嗜血杆菌的检出率明显高于纸片法卫星试验,差异有统计学意义(P<0.05),而与API生化细菌鉴定系统比较检出率差异无统计学意义(P>0.05)。MALDI-TOP MS检测所用时间为5~10 min,API生化细菌鉴定检测所用时间为4~24 h,纸片法卫星试验检测所用时间为24~48 h。采用MALDI-TOP MS鉴定流感嗜血杆菌的成本更低,传统API生化细菌鉴定方法价格昂贵,而纸片法卫星试验操作比较繁琐。结论MALDI-TOP MS检测系统对流感嗜血杆菌鉴定的准确率较高,鉴定周期较短,成本较低,且使用方便,在临床上对流感嗜血杆菌的检出具有重要价值。Objective To analyze the application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOP MS)in the identification of Haemophilus influenzae.Methods The 1300 samples of sputum or throat swab received in the laboratory of Nanjing Aidikang Medical Laboratory Co.,Ltd.from January to December,2022 were collected from respiratory infection patients,from which Haemophilus influenzae was cultivated and isolated.Using 16SrDNA sequencing,50 strains of Haemophilus influenzae were detected.The MALDI-TOP MS detection system,Analytical Products INC(API)biochemical bacterial identification system and paper method satellite experiment were used to identify Haemophilus influenzae and the detectable rate,detection time,detection cost and reading method of above three methods were compared.Results The detectable rate of Haemophilus influenzae by MALDI-TOP MS was 98.0%,the detectable rate of API biochemical bacterial identification system was 90.0%,and the detectable rate of paper disk satellite test was 74.0%.The detectable rate of Haemophilus influenzae by MALDI-TOP MS was higher than that of paper disk method satellite test,and the difference was statistically significant(P<0.05).However,compared with API biochemical bacterial identification system,the difference was not statistically significant(P>0.05).The time required for MALDI-TOP MS detection was 5-10 minutes,the time required for API biochemical bacterial identification detection was 4-24 hours,and the time required for paper disk satellite test detection was 24-48 hours.The cost of using MALDI-TOP MS to identify Haemophilus influenzae was lower,traditional API biochemical identification method was expensive,and the paper disk method satellite test operation was cumbersome.Conclusions The MALDI-TOP MS detection system has high accuracy,short identification cycle and low cost in identifying Haemophilus influenzae,and is easy to use.It has important value in the clinical detection of Haemophilus influenzae.

关 键 词：基质辅助激光解吸电离飞行时间质谱 流感嗜血杆菌 Analytic Products INC生化细菌鉴定系统 纸片法卫星试验 

分 类 号：R446.5[医药卫生—诊断学]