高粱×苏丹草后代群体农艺及产量性状QTL初步定位  

QTL Preliminary Mapping of Agronomic and Yield Traits in Sorghum×Sudan grass Progeny Population

作　　者：孙安栋 高建明 吕芃[2] 裴忠有 杨庭金 罗峰 SUN Andong;GAO Jianming;L Peng;PEI Zhongyou;YANG Tingjin;LUO Feng(College of Agronomy,Resources and Environment,Tianjin Agricultural University,Tianjin Key Laboratory of Intelligent Breeding of Major Crops,Tianjin 300392,China;Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences,Hebei Branch of National Sorghum Improvement Center,Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Key Laboratory of Minor Cereal Crops of Hebei Province,Shijiazhuang 050035,China)

机构地区：[1]天津农学院农学与资源环境学院,天津市主要农作物智能育种重点实验室,天津300392 [2]河北省农林科学院谷子研究所,国家高粱改良中心河北分中心,农业农村部特色杂粮遗传改良与利用重点实验室(部省共建),河北省杂粮研究实验室,河北石家庄050035

出　　处：《华北农学报》2024年第5期15-23,共9页Acta Agriculturae Boreali-Sinica

基　　金：天津市主要农作物智能育种重点实验室青年开放课题(KLIBMC2307);天津市优秀科技特派员项目(23ZYCGSN00150)。

摘　　要：为进一步探究高粱籽粒和茎秆产量的遗传规律,运用粒用高粱忻粱52和苏丹草TS 185作为亲本进行杂交并得到F_(2)及F_(2)∶3群体,利用115对多态性引物对430份F_(2)群体使用区间作图法构建遗传连锁图谱,以LOD值等于3作为阈值。结果表明,分蘖数、叶片数、茎粗、穗长、株高、茎秆鲜质量、整株鲜质量、着壳率、穗质量、千粒质量、单穗粒质量、单穗粒数12个农艺性状共检测到86个QTL。在1号染色体sam17164—sam15397区间定位到茎秆鲜质量的QTL;在2号染色体Xcup64—Xcup26区间定位到分蘖数的QTL,Xtxp019—sam01138区间定位到叶片的QTL,Xcup26—Xtxp080区间定位到茎秆鲜质量的QTL;在3号染色体sam44791—sam33751区间定位到穗长的QTL;在7号染色体sam39622—sam43980区间定位到着壳率的QTL;在8号染色体sam10491—sam17740区间定位到整株鲜质量的QTL;在10号染色体sam710901b—sam59778区间定位到分蘖数的QTL,以上这些都是新检测到的位点。In order to further explore the genetic rule of grain and stalk yield of sorghum,grain sorghum Xinliang 52 and Sudan grass TS 185 were hybridized as parents to obtain F_(2) and F_(2)∶3 populations,115 pairs of polymorphic primers were used to construct the genetic linkage map of 430 F_(2) progeny populations by interval mapping.A total of 86 QTLs were detected for 12 agronomic traits,including number of tillers,number of blades,stem diameter,panicle length,plant height,stem fresh weight,whole plant fresh weight,shelling rate,panicle weigh,thousand kernels weight,grain weight per panicle and grain number per panicle,with LOD value as the threshold value.The QTL of stem fresh weight was located between sam17164—sam15397 on chromosome 1.On chromosome 2,the QTL of number of tillers was located in Xcup64—Xcup26 region,leaf was located in Xtxp019—sam01138 region,and stem fresh weight was located in Xcup26—Xtxp080 region.Panicle length QTL was located between sam44791 and sam33751 on chromosome 3.The QTL of shelling rate was located at sam39622—sam43980 on chromosome 7.The QTL for whole plant fresh weight located in sam10491—sam17740 on chromosome 8 and the QTL for number of tillers located in sam710901b—sam59778 on chromosome 10 were all newly detected loci.

关 键 词：高粱 SSR标记 数量性状位点 QTL定位 

分 类 号：S514[农业科学—作物学]