布鲁氏菌病四种检测方法的比较分析  

Comparative Analysis of Four Detection Methods for Brucellosis

作　　者：马爱霞 段佳燚 朱伟宁 张新亮 MAAi-xia;DUAN Jia-yi;ZHU Wei-ning;ZHAGN Xin-liang(Shandong Vocational Animal Science and Veterinary College,Weifang 261061)

机构地区：[1]山东畜牧兽医职业学院,潍坊261061

出　　处：《中国奶牛》2024年第10期33-36,共4页China Dairy Cattle

基　　金：山东省农业重大技术协同推广计划项目(SDNYXTTG-2023-07);潍坊市科技发展计划项目(2021GX030)。

摘　　要：为了研究能快速准确诊断布鲁氏菌病的实验室检测方法,将使用布鲁氏菌病虎红平板凝集试验(RBT)进行初筛的来自两个牛场的血清样品,分别用试管凝集试验(SAT)、间接酶联免疫吸附试验(iELISA)、竞争酶联免疫吸附试验(cELISA)4种不同的血清学检测方法进行检测,比较四种方法的特异性、敏感性、符合率、Kappa值等试验指标,分析各方法在布鲁氏菌病诊断中的差异,找出适合初筛和确诊的实验室诊断的方法。结果显示,RBT、iELISA的敏感性高于SAT和cELISA,RBT与cELISA特异性和符合率较高。所以对于布鲁氏菌病实验室诊断,要结合实际条件选择检测方法,RBT与iELISA适合用于初筛,SAT与cELISA适合确诊,积极推广对防控动物布鲁氏菌病意义重大。In order to study the laboratory detection method for rapid and accurate diagnosis of brucellosis.The serum samples from two cattle farms screened by brucellosis tiger red plate agglutination test(RBT)were tested by test tube agglutination test(SAT),indirect enzyme linked immunosorbent assay(iELISA)and competitive enzyme linked immunosorbent assay(cELISA).The specificity,sensitivity,coincidence rate and Kappa value of the four methods were compared.The differences of various methods in the diagnosis of brucellosis were analyzed to find out the methods suitable for primary screening and laboratory diagnosis.The results showed that the sensitivity of RBT and iELISA was higher than that of SAT and cELISA,RBT and the specificity and coincidence rate of cELISA were higher.Therefore,for the laboratory diagnosis of brucellosis,the detection methods should be selected according to the actual conditions,RBT and iELISA are suitable for primary screening,SAT and cELISA are suitable for diagnosis,active promotion is of great significance for the prevention and control of animal brucellosis.

关 键 词：布鲁氏菌病 虎红平板凝集试验 试管凝集试验 酶联免疫吸附试验 比较分析 

分 类 号：S858.23[农业科学—临床兽医学]