黑木耳遗传多样性分析及高产多糖菌株筛选  

Genetic Diversity Analysis and Strains Screening of Auricularia auricula with High Polysaccharide Yield

作　　者：李红[1] 刘岩岩[1] 刘俊杰[1] 曹君[1] LI Hong;LIU Yanyan;LIU Junjie;CAO Jun(Edible Fungi Institute,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China)

机构地区：[1]辽宁省农业科学院食用菌研究所,辽宁沈阳110161

出　　处：《中国食用菌》2024年第5期25-34,53,共11页Edible Fungi of China

基　　金：辽宁省科学技术厅辽宁省自然科学基金面上资助项目(2022-MS-057);辽宁省新宾县食用菌科技特派团资助项目(2023JH5/10400122)。

摘　　要：利用体细胞不亲和性分析、ISSR分子标记对东北三省地区主栽及收集的45份黑木耳种质资源进行遗传多样性分析,并通过液体深层发酵技术筛选高产多糖菌株。结果表明,其中9个菌株拮抗反应不明显,亲缘关系较近;其他36个菌株的遗传相似系数变化范围为0.44~0.96,遗传背景丰富。当遗传相似系数为0.6时,供试菌株聚类为9个类群:黑山、黑丰、黑丰2、神8-7、黑威伴金、黑威15、黑保1号、2016-A、2016-B、2016-C、2016-E、2019-013、2019-192、新科10号、黑厚圆、华腾2号、华腾4号、春宝为第1类群;木29、长白7、新科1号、919为第2类群;高优王、1022、2019-003、918为第3类群;蕈丰黑、延丰13、半筋S1908、888、丰产王为第4类群;靖发13、89-1、丰收2号、2019-199、海元的遗传相似系数小于0.6,亲缘关系较远,单个菌株自成一类,分别为第5~9类群。经筛选,得到1株多糖含量高且丰产的黑木耳菌株黑丰2。The genetic diversity of 45 Auricularia auricula germplasm resources collected from three northeastern provinces was analyzed by somatic incompatibility analysis and ISSR molecular marker,then high yield polysaccharide strains of which were screened by the liquid deep fermentation technology.The results showed that 9 strains had no obvious antagonistic reaction and were closely related to each other.The genetic similarity coefficient of the other 36 strains ranged from 0.44 to 0.96,and the genetic background was rich.When the genetic similarity coefficient was 0.6,the tested strains were clustered into nine groups:Heishan,Heifeng,Heifeng 2,Shen 8-7,Heiwei Banjin,Heiwei-15,Heibao No.1,2016-A,2016-B,2016-C,2016-E,2019-013,2019-192,Xinke No.10,Heihouyuan,Huateng No.2,Huateng No.4 and Chunbao were the first group;Mu 29,Changbai 7,Xinke No.1 and 919 were the second group;Gaoyouwang,1022,2019-003 and 918 were the third group;Xunfenghei,Yanfeng-13,Banjin S1908,888 and Fengchanwang were the fourth group;The genetic similarity coefficient of Jingfa 13,89-1,Fengshou No.2,2019-199 and Haiyuan was less than 0.6,indicating that the genetic relationship was distant,and the single strain as sui generis,which were the fifth to the ninth groups respectively.From these strains,A.auricula strain Heifeng 2 with high polysaccharide content and high yield was screened out.

关 键 词：黑木耳 ISSR分子标记 遗传多样性 多糖 东北三省 

分 类 号：S646.6[农业科学—蔬菜学]