干巴菌菌丝体与原基的比较转录组学分析  

Comparative Transcriptomic Analysis of Mycelia and Primordium of Thelephora ganbajun Zang

作　　者：李鹏宇 胡骐验 邵娅丽 杨正一 赵梦婷 胡瑛琳 丁梓洲 沙涛[1] LI Pengyu;HU Qiyan;SHAO Yali;YANG Zhengyi;ZHAO Mengting;HU Yinglin;DING Zizhou;SHA Tao(State Key Laboratory for Conservation and Utilization of Bio-Resource in Yunnan,School of Life Sciences,Yunnan University,Kunming 650091,China;Yunnan Institute of Population and Family Planning Science and Technology,Kunming 650032,China)

机构地区：[1]云南大学生命科学学院省部共建云南生物资源保护与利用国家重点实验室,云南昆明650091 [2]云南省人口和计划生育科学技术研究所,云南昆明650032

出　　处：《中国食用菌》2024年第5期58-66,共9页Edible Fungi of China

基　　金：国家自然科学基金项目(No.31260006);2022年云南大学“双一流”建设进化生物学与前沿交叉领军人才队伍建设项目。

摘　　要：干巴菌是云南省一种重要的野生食用真菌,但其发育过程的相关分子机制尚未被揭示。研究旨在以干巴菌G46号菌株基因组作为参考基因组,对干巴菌菌丝体、干巴菌原基进行转录组测序及有参分析。结果表明,差异表达基因(DEG)总数为4197个,2051个上调,2146个下调。GO富集分析表明,DEG在生物过程的多个条目中呈现出显著的富集现象,具体包括代谢过程、细胞过程以及单有机体过程等;在细胞组分的分类中,DEG显著富集于细胞、细胞部分、细胞器部分等条目;在分子功能的层面上,DEG还显著富集于催化活性、结合、转运体等关键条目中。这一发现揭示了DEG在生物体内具有广泛而多样的功能和作用。KEGG通路富集分析结果显示,DEG主要富集于MAPK信号通路、内质网中的蛋白质加工、氨基糖和核苷酸糖代谢通路等。内质网中的蛋白质加工、氨基糖和核苷酸糖代谢等途径相关DEG在原基期大部分上调表达。此外,共1742个碳水化物活性酶(CAZymes)相关DEG表达,其中碳水化合物结合模块(CBMs)与糖苷水解酶(GHs)的DEG数量最多,分别占40.01%和29.56%。这些基因主要参与糖类底物降解,表明原基形成需要大量物质和能量。同时,降解木质纤维素的基因富集,暗示干巴菌的原基发育可能与其共生植物密切相关。Thelephora sp.,an esteemed wild edible fungus in Yunnan Province,has yet to fully disclose its molecular developmental mechanisms.Leveraging the G46 genome of Thelephora ganbajun Zang as a reference,transcriptome sequencing and analysis were conducted on its mycelium and primordium.The analysis uncovered 4197 differentially expressed genes(DEG),with 2051 upregulated and 2146 downregulated.GO enrichment analysis highlighted significant enrichment of these DEG in biological processes related to metabolism,cellular processes,and single-organism activities;cellular components including cell,cell part,and organelle part;and molecular functions such as catalytic activity,binding,and transporter activity.KEGG pathway enrichment analysis further revealed that the MAPK signaling pathway,protein processing in the endoplasmic reticulum,and amino sugar and nucleotide sugar metabolism pathways were enriched among the DEG.Notably,DEG involved in protein processing in the endoplasmic reticulum and amino sugar and nucleotide sugar metabolism were predominantly upregulated and expressed during the prophase.Additionally,the expression of 1742 DEG associated with carbohydrate-active enzymes(CAZymes)was observed,with the carbohydrate-binding modules(CBMs)and glycoside hydrolases(GHs)families being the most abundant,accounting for approximately 40.01%and 29.56%respectively.These genes played a pivotal role in sugar substrate degradation,indicating a substantial demand for materials and energy during primordium formation.The enrichment of genes associated with lignocellulose degradation also suggests a potential close relationship between the development of T.ganbajun primordia and its symbiotic plants.

关 键 词：干巴菌 原基形成 转录组 生长发育 外生菌根真菌 

分 类 号：S646.2[农业科学—蔬菜学]