野生大型真菌鉴定及其木质纤维素酶对玉米秸秆降解效果的影响  

Identification of Wild Macrofungi and the Effect of Lignocellulase On the Degradation Effect of Corn Stover

作　　者：柳卓含 律凤霞[1] LIU Zhuohan;LYU Fengxia(Department of Biology,Mudanjiang Normal College,Mudanjian 157011,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《中国食用菌》2024年第5期80-85,共6页Edible Fungi of China

基　　金：国家自然科学基金项目(31700587);牡丹江师范学院揭榜挂帅项目(MSYSYL2022016)。

摘　　要：为筛选野生大型真菌中对作物秸秆有显著降解效果的腐解菌,秋季多雨季节在牡丹峰国家森林公园野外采集了15种大型真菌子实体,提取其子实体DNA经ITS序列鉴定遗传种属,确定了12株野生大型真菌隶属于10个科、12个属。再经组织分离法获得9株菌丝纯培养物。以木质纤维素酶活为指标筛选得到4株高木质纤维素酶活菌株;筛选后菌株代谢液按比例添加至玉米秸秆碎段进行常温高湿发酵降解,以玉米秸秆失重率及木质纤维素降解率为测定指标验证了试验中香栓菌对玉米秸秆的降解效果更好。In order to screen the decomposition fungi that have a significant degradation effect on crop straw in wild macrofungi,15 species of macrofungal fruit bodies were collected in the field of Mudanfeng National Forest Park in the rainy season in autumn,and the DNA of their fruit bodies was extracted and the genetic species were identified by ITS sequences,and 12 wild macrofungi strains were determined belonging to 10 families and 12 genera.9 pure mycelial cultures were obtained by tissue separation.4 live strains with high lignocellulase were screened by using lignocellulase activity as the index.After screening,the metabolic solution of the strain was added to the corn stover crushing section for fermentation and degradation at room temperature and high humidity,and the weight loss rate and lignocellulase degradation rate of corn stover were used as the measurement indexes to verify that the degradation effect of Trametes suaveloens in the experiment was the better.

关 键 词：野生大型真菌 香栓菌 木质纤维素酶 组织分离 玉米秸秆降解 

分 类 号：S646.9[农业科学—蔬菜学]