消痰化瘀利窍方对慢性间歇性低氧小鼠胸主动脉内皮细胞损伤的保护作用  

Protective Effects of Xiaotan Huayu Likiao Formula on Thoracic Aortic Endothelial Cell Injury in Mice with Chronic Intermittent Hypoxia

作　　者：张睦清[1] 李红京 张晓雪 丁哲 郭秋红 ZHANG Mu-qing;LI Jing-hong;ZHANG Xiao-xue;ZHANG Na-bo;GUO Qiu-hong(Hebei Hospital of Traditional Chinese Medicine,Shijiazhuang,050011,China;Hebei University of Chinese Medicine,Shijiazhuang,050200,China;Hebei Chinese Medicinal Formulation Technology Innovation Center/Hebei University Chinese Medicinal Development and Industrialization Application Technology Research and Development Center,Shijiazhuang,050200,China)

机构地区：[1]河北省中医院,石家庄050011 [2]河北中医药大学,石家庄050200 [3]河北省中药组方制剂技术创新中心/河北省高校中药开发与产业化应用技术研发中心,石家庄050200

出　　处：《河北中医药学报》2024年第5期1-5,共5页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省重点研发计划项目民生科技专项:No.192777104D。

摘　　要：目的:探讨消痰化瘀利窍方(Xiaotan Huayu Liqiao Formula,XHLF)改善慢性间歇性低氧(Chronic Intermittent Hypoxia,CIH)小鼠胸主动脉内皮细胞的损伤作用机制。方法:将24只健康的8周龄雄性C57BL/6N小鼠随机分成常氧组(Con组)、慢性间歇性低氧暴露组(CIH组)、慢性间歇性低氧+消痰化瘀利窍方组(CIH+XHLF组)、常氧+消痰化瘀利窍方组(Con+XHLF组)。CIH组与CIH+XHLF组暴露于间歇性低氧的环境中,CIH+XHLF组和Con+XHLF组每日给予消痰化瘀利窍方灌胃(26.8 g/kg/d),而CIH组与Con组给予同等体积生理盐水,共计处理28 d。采用尾套法监测小鼠血压;HE和Masson染色观察胸主动脉病理改变;检测血清中一氧化氮(NO)、血管内皮生长因子(VEGF)含量;透射电镜观察线粒体形态;Tunel染色观察胸主动脉细胞凋亡情况;免疫组织化学染色检测Cleaved Caspase-3、Bcl-2相关X蛋白(Bax)、B淋巴细胞瘤-2基因(Bcl-2)蛋白表达。结果:与Con组相比,CIH组小鼠血压出现升高(P<0.05);血管内膜不平整,细胞核排列紊乱,弹性纤维减少;血清中VEGF水平升高,NO水平下降(P<0.05);线粒体皱缩、嵴断裂;凋亡水平增加,Cleaved Caspase-3、Bax蛋白表达增强,Bcl-2蛋白表达减弱(P<0.05)。与CIH组相比,CIH+XHLF组小鼠血压下降(P<0.05),血管内皮功能和损伤改善(P<0.05),血管内皮线粒体损伤改善,凋亡水平下降(P<0.05)。结论:XHLF可能通过抑制凋亡改善CIH暴露引起的胸主动脉血管损伤和功能障碍。Objective:To investigate the protective mechanism of Xiaotan Huayu Likiao Formula(XHLF)in improving thoracic aortic endothelial cell injury in mice with chronic intermittent hypoxia(CIH).Methods:Twenty-four healthy male C57BL/6N mice aged 8 weeks were randomly divided into four groups:normoxia group(Con),chronic intermittent hypoxia exposure group(CIH),CIH+XHLF group,and Con+XHLF group.The CIH group and the CIH+XHLF group were exposed to an intermittent hypoxic environment.The CIH+XHLF and Con+XHLF groups were administered XHLF daily by gastric lavage(26.8 g/kg/d),while the CIH and Con groups were given an equal volume of normal saline for a total of 28 days.The tail-cuff method was used to monitor the mouse blood pressure;HE and Masson staining to observe the pathological changes in the thoracic aorta;The serum levels of nitric oxide(NO)and vascular endothelial growth factor(VEGF)were measured.Mitochondrial morphology was observed under transmission electron microscopy.TUNEL staining was used to detect apoptosis of thoracic aortic cells;Immunohistochemical staining to detect the expression of Cleaved Caspase-3,Bcl-2-associated X protein(Bax),and B-cell lymphoma-2 gene(Bcl-2)proteins.Results:Compared with the Con group,the systolic blood pressure of mice in the CIH group increased after 21 days(P<0.05).The vascular intima was uneven,the nuclear arrangement was disordered,and the elastic fibers were reduced.The level of VEGF in the serum increased,while the level of NO decreased(P<0.05).Mitochondria showed shrinkage and cristae fracture.The apoptosis level increased,and the expressions of Cleaved Caspase-3 and Bax proteins were enhanced,while the expression of Bcl-2 protein was weakened(P<0.05).Compared with the CIH group,the blood pressure of mice in the CIH+XHLF group decreased(P<0.05),and vascular endothelial function and injury improved(P<0.05).Vascular endothelial mitochondrial damage was improved,and the apoptosis level decreased(P<0.05).Conclusion:XHLF may improve thoracic aortic vascular injury and dysfunc

关 键 词：消痰化瘀利窍方 慢性间歇性低氧 胸主动脉 内皮细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]