Isolation and characterization of 16 microsatellite loci from transcriptome-derived sequences of the topmouth culter(Culter alburnus Basilewsky)  

作　　者：Shili Liu Yinuo Liu Jianbo Zheng Fei Li Wenping Jiang Meili Chi Shun Cheng Yongyi Jia Jinliang Zhao 

机构地区：[1]Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture,Shanghai Ocean University,Shanghai,201306,China [2]Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai,201306,China [3]National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai,201306,China [4]Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province,Zhejiang Institute of Freshwater Fisheries,Huzhou,313001,China

出　　处：《Aquaculture and Fisheries》2024年第5期739-744,共6页渔业学报（英文）

基　　金：supported by the Major science and technology programs for agriculture (aquaculture)new variety breeding in Zhejiang province (2021C02069-3).

摘　　要：The topmouth culter(Culter alburnus)is at the top of the food chain and plays an important role in the maintenance of freshwater ecosystem stability.It is also a commercially important aquaculture fish species in China.To establish conservation strategies and breeding programs,a total of 16 polymorphic microsatellite loci were isolated from transcriptome-derived sequences of C.alburnus.Characterization of these loci was assessed against 1 wild and 3 cultured populations in Huzhou,China.The number of alleles per locus ranged from 3 to 10 with an average of 5.25.A bottleneck effect was observed in all 4 populations;The ChangXing population showed moderate genetic differentiation when compared with other populations(FST values,0.0695-0.0788).The microsatellite loci would be useful for further studies on population genetic diversity,genetic structure,and assessments of the artificial propagation release effect of C.alburnus.

关 键 词：Culter alburnus MICROSATELLITE Genetic diversity 

分 类 号：S917[农业科学—水产科学]