Transcriptomic analysis of regenerated skins reveals key genes involved in skin immune response and air-breathing of loach(Paramisgurnus dabryanus)  

作　　者：Rongyun Li Jian Gao Yunbang Zhang Bing Sun Xiaojuan Cao 

机构地区：[1]College of Fisheries,Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Huazhong Agricultural University,Wuhan,430070,China [2]Hubei Provincial Engineering Laboratory for Pond Aquaculture,Wuhan,430070,China

出　　处：《Aquaculture and Fisheries》2024年第5期753-762,共10页渔业学报（英文）

基　　金：supported by the National Natural Science Foundation of China (32172962 and 31872579);the Fundamental Research Funds for the Central Universities of China (Project Number:2662020SCPY002).

摘　　要：Fish skin acts as the first immune barrier against pathogens from external environments.Recently,fish skin immunity research has gradually become a hot topic in aquaculture disease control.Interestingly,the skin can do air-breathing in some bimodal respiration fish.However,there is no comprehensive understanding of these two functions.In this study,the skin regeneration of loach(Paramisgurnus dabryanus)was investigated through morphological and histological observations.Then,original skins(OS)and the regenerated skins(RS)when their capillaries were the most abundant during healing,were collected for transcriptomic analysis.285,899,386 clean reads with a total length of 42.34 Gb were obtained.A total of 1282 differentially expressed genes(DEGs)were detected,including 1030 DEGs up-regulated and 252 DEGs down-regulated in the comparison of RS vs.OS.Fc gamma R-mediated phagocytosis(containing gelsolin(Gsn)),chemokine signaling pathway(containing C-C motif chemokine ligand 28(Ccl28)),and B cell receptor signaling pathway(containing CD80 molecule(Cd81))were closely related to skin immune response of the loach.PI3K-Akt signaling pathway[containing fibronectin 1(Fn1)and lysyl oxidase-like 2b(Loxl2b)],TGF-beta signaling pathway[containing thrombospondin 1(Thbs1)],ECM-receptor interaction[containing integrin alpha 7(Itga7),Itgb8,Itgb10 and Itgb5],and dilated cardiomyopathy[containing laminin subunit alpha1(Lama1)]were closely associated with skin air-breathing of the loach.This study is conducive to explore the molecular mechanisms of skin immune response during its regeneration and air-breathing of bimodal respiration fish.This study will benefit for the aquaculture of P.dabryanus and its related species.

关 键 词：Paramisgurnus dabryanus Skin regeneration Transcriptomic analysis Skin air-breathing Skin immune response 

分 类 号：S917[农业科学—水产科学]