免疫荧光检测石蜡切片IgA蛋白在诊断疱疹样皮炎和线状IgA大疱性皮病的应用评价  

Assessment of IgA protein in paraffin sections by immunofluorescence in the diagnosis of dermatitis herpetiformis and linear IgA bullous dermatitis

作　　者：王建文 刘永霞[1,2] 陈祚玺 陈学超[1,2] 周桂芝 王田宇 陈声利[1,2] 孙勇虎 张福仁[1,2,3] WANG Jianwen;LIU Yongxia;CHEN Zuoxi;CHEN Xuechao;ZHOU Guizhi;WANG Tianyu;CHEN Shengli;SUN Yonghu;ZHANG Furen(Hospital for Skin Diseases,Shandong First Medical University,Jinan 250022,China;Shandong Provincial Institute of Dermatology and Venereology,Shandong Academy of Medical Sciences,Jinan 250022,China;University of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东第一医科大学附属皮肤病医院,山东济南250022 [2]山东省皮肤病性病防治研究所,山东济南250022 [3]山东中医药大学,山东济南250014

出　　处：《中国麻风皮肤病杂志》2024年第12期847-850,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金面上项目(编号:82073441);山东省泰山学者青年专家项目(编号:tsqn201909141)。

摘　　要：目的:评价直接免疫荧光染色检测石蜡切片IgA抗体对于诊断疱疹样皮炎(DH)和线状IgA大疱性皮病(LABD)的应用价值。方法:选取2019-2024年我院病理诊断的DH和LABD病例,入选实验组病例DH 46例,LABD 32例。采用FITC荧光标记兔抗人IgA抗体进行直接免疫荧光染色检测。结果:46例DH石蜡切片中,40例IgA在真皮乳头为阳性,阳性率86.96%。32例LABD石蜡切片中,20例IgA在表皮基底膜线状沉积为阳性,阳性率62.50%。结论:直接免疫荧光染色检测石蜡切片IgA可以作为辅助诊断DH和LABD的有效方法。Objective:To evaluate the application value of direct immunofluorescence staining for detecting IgA antibodies in paraffin sections in the diagnosis of dermatitis herpetiformis(DH)and linear IgA bullous dermatosis(LABD).Methods:DH and LABD cases diagnosed pathologically in our hospital from 2019 to 2024 were selected,including 46 cases of DH and 32 cases of LABD.Direct immunofluorescence staining was performed using FITC-labeled rabbit anti-human IgA antibody.Results:Among the 46 paraffin sections of DH patients,IgA was positively deposited in the dermal papillae in 40 of them,with a positivity rate of 86.96%.Among the 32 paraffin sections of LABD patients,IgA was positively deposited linearly in the epidermal basement membrane in 20 of them,with a positivity rate of 62.50%.Conclusion:Direct immunofluorescence staining for detecting IgA in paraffin sections can be used as an effective method for assisting in the diagnosis of DH and LABD.

关 键 词：疱疹样皮炎 线状IGA大疱性皮病 石蜡切片 免疫荧光 IgA 

分 类 号：R758.66[医药卫生—皮肤病学与性病学]