不同时间按压刺激对大鼠骨骼肌形态学及肿瘤坏死因子α、核因子κB的影响  

Effects of pressor stimulation at different times on rat skeletal muscle morphology and tumor necrosis factor alpha and nuclear factor kappaB

作　　者：石培丽 林森 赵文腾 彭亚力 胡亚哲[1] Shi Peili;Lin Sen;Zhao Wenteng;Peng Yali;Hu Yazhe(School of Physical Education and Sports,Central China Normal University,Wuhan 430079,Hubei Province,China;School of Sports Science and Technology,Wuhan Sports University,Wuhan 430205,Hubei Province,China)

机构地区：[1]华中师范大学体育学院,湖北省武汉市430079 [2]武汉体育学院体育科技学院,湖北省武汉市430205

出　　处：《中国组织工程研究》2025年第17期3588-3595,共8页Chinese Journal of Tissue Engineering Research

基　　金：华中师范大学科研发展基金(20206982475),项目负责人:胡亚哲。

摘　　要：背景:研究发现不同时间长度按压点刺激正常肌肉,将产生不同的生理反应。目的:探讨在不同时间机械压力按压下,大鼠骨骼肌内炎性因子肿瘤坏死因子α及核因子κB表达的变化。方法:健康SPF级SD雄性大鼠20只,随机分为空白对照组、10 s按压组、20 s按压组及30 s按压组,每只大鼠的单侧右腿用于实验。空白对照组不予干预,各按压组大鼠经2%戊巴比妥钠(35 mg/kg)腹腔注射麻醉后,使用自制机械压力装置,将压强固定在200 kPa,分别持续按压大鼠股薄肌10,20,30 s,随后即刻取右后肢按压处肌肉组织。苏木精-伊红染色观察骨骼肌组织形态学变化及肌纤维横截面积的变化,免疫组织化学检测骨骼肌肿瘤坏死因子α及核因子κB表达水平。结果与结论:①苏木精-伊红染色结果显示,各按压组大鼠骨骼肌肌纤维排列松散,肌纤维横截面积和直径变小,骨骼肌间隙变大。与空白对照组相比,各按压组骨骼肌肌纤维的横截面积显著减小(P<0.05),3个按压组之间骨骼肌肌纤维的横截面积差异无显著性意义(P>0.05)。10 s按压组肌纤维间隙中未见明显红细胞,20 s按压组间隙中出现少量红细胞,30 s按压组骨骼肌肌纤维间毛细血管扩张,间隙中出现红细胞。②30 s按压组的骨骼肌肿瘤坏死因子α的表达水平显著高于空白对照组(P<0.05)。③各组间的骨骼肌核因子κB的表达水平比较差异无显著性意义(P>0.05)。④结果说明,压强固定在200 kPa时,骨骼肌经过按压会发生形态学变化,肌纤维横截面积减少,但按压10,20,30 s骨骼肌形态学及骨骼肌纤维横截面积无显著差异。随着时间点的变化,按压骨骼肌至30 s时,炎性因子肿瘤坏死因子α启动,但对核因子κB没有影响,推测短时间内炎性因子可出现表达,而转录因子未见明显变化。BACKGROUND:Studies have shown that different durations of pressure application on normal muscles can produce varying physiological responses.OBJECTIVE:To explore the expression levels of inflammatory factors tumor necrosis factorαand nuclearκB in skeletal muscle under different pressure durations.METHODS:Twenty healthy male SPF-grade Sprague-Dawley rats were randomly divided into four groups:control group,10-second pressure group,20-second pressure group,and 30-second pressure group.The right leg of each rat was used for the experiment.The control group received no intervention,while rats in each pressure group were anesthetized by intraperitoneal injection of 2%pentobarbital sodium(35 mg/kg),and the thin femoral muscle of the rats was pressed continuously at a constant pressure of 200 kPa using a homemade mechanical pressure device for 10,20,and 30 seconds,respectively.Muscle tissue at the pressing site of the right hind limb was collected immediately after pressure.Hematoxylin-eosin staining was used to observe the morphological changes of skeletal muscle tissues and changes in the cross-sectional area of muscle fibers,and immunohistochemistry was used to detect the expression levels of tumor necrosis factorαand nuclear factorκB in rat skeletal muscle.RESULTS AND CONCLUSION:Hematoxylin-eosin staining results revealed that the pressure groups showed loosely arranged skeletal muscle fibers,reduced cross-sectional area and diameter,and enlarged intermuscular spaces.Compared with the control group,the cross-sectional area of muscle fibers was significantly reduced in the pressure groups(P<0.05),but there was no significant difference between the three pressure groups(P>0.05).The 10-second pressure group showed no significant presence of red blood cells in the interstitial spaces,while the 20-second pressure group exhibited a small amount of red blood cells,and the 30-second pressure group showed capillary dilation with red blood cells in the interstitial spaces.The expression level of tumor necrosis factorαin

关 键 词：骨骼肌 炎性因子 肿瘤坏死因子Α 核因子ΚB 工程化细胞因子 工程化组织构建 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R446