有氧运动促进肌少性肥胖小鼠骨骼肌能量代谢通路各环节的适应性变化  

Aerobic exercise promotes remodeling of the energy metabolism network in the skeletal muscle of mice with sarcopenic obesity

作　　者：陈聪 吴慧娟 胡悦[1,4] 周黄浩 黄春秀 Chen Cong;Wu Huijuan;Hu Yue;Zhou Huanghao;Huang Chunxiu(National-Local Joint Engineering Research Center of Rehabilitation Medicine Technology,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian Province,China;Rehabilitation Industry Institute,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian Province,China;Fujian Key Laboratory of Cognitive Rehabilitation,Fuzhou 350122,Fujian Province,China;School of Rehabilitation Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian Province,China)

机构地区：[1]福建中医药大学康复医疗技术国家地方联合工程研究中心,福建省福州市350122 [2]福建中医药大学康复产业研究院,福建省福州市350122 [3]福建省认知功能康复重点实验室,福建省福州市350122 [4]福建中医药大学康复医学院,福建省福州市350122

出　　处：《中国组织工程研究》2025年第17期3596-3604,共9页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(32271175),项目负责人:陈聪;福建省自然科学基金项目(2021J01960),项目负责人:陈聪。

摘　　要：背景:肌少性肥胖是一种肌少症和肥胖共存的疾病,患病率持续上升。有氧运动可缓解肌少性肥胖进程,但运动后骨骼肌能量代谢的整体变化仍不明晰。目的:探究有氧运动对肌少性肥胖小鼠骨骼肌细胞代谢通路各个环节适应性变化的影响。方法:将C57BL/6J雄性小鼠随机分为正常对照组和造模组,分别喂养普通饲料和高脂饲料12周后,通过体质量和行为学筛选出肌少性肥胖小鼠模型。随后,采用随机数字表法将造模组小鼠分为静坐组和运动组,运动组在高脂喂养基础上进行有氧跑台训练。干预8周后,检测小鼠体质量、脂质代谢、后肢小腿肌群肌肉体积、骨骼肌形态和能量代谢通路相关基因表达水平。结果与结论:①与正常对照组相比,静坐组小鼠血清中三酰甘油、总胆固醇和游离脂肪酸水平、肌内脂滴沉积显著增加(P<0.05);与静坐组相比,运动组上述指标均呈现显著下降趋势(P<0.05);②与正常对照组相比,静坐组小鼠抓力下降,转棒停留时间明显缩短,后肢小腿肌群肌肉体积和肌纤维横截面积明显减小(P<0.05),肌内Atrogin-1和Murf-1 mRNA表达升高(P<0.05);与静坐组相比,运动组小鼠抓力、转棒停留时间、后肢小腿肌群肌肉体积、肌纤维横截面积均得到明显改善(P<0.05),Atrogin-1和Murf-1 mRNA水平降低(P<0.05);③与正常对照组比,静坐组小鼠肌内转录因子Pparα、Pgc-1αmRNA表达降低(P<0.05),脂肪酸合成相关酶Srebp1c、Fasn mRNA表达升高(P<0.05),β氧化体系Cpt1β、Acox1、Acox3和脂肪酸代谢因子Arf1、Plin3 mRNA表达降低(P<0.05);与静坐组相比,运动组上述基因的异常表达得以明显逆转(P<0.05);④结果表明,有氧运动通过调节肌内能量代谢网络基因表达,缓解脂质沉积,改善肌肉质量和力量。BACKGROUND:Sarcopenia obesity is characterized by the coexistence of sarcopenia and obesity with a continuously increasing prevalence.Aerobic exercise can alleviate the progression of sarcopenic obesity,but the overall metabolic changes in skeletal muscle after exercise are still unclear.OBJECTIVE:To investigate the effects of exercise on various aspects of the energy metabolic pathways in the skeletal muscle of mice with sarcopenic obesity.METHODS:Male C57BL/6J mice were randomly divided into normal control(normal diet)and model(high-fat diet)groups.After 12 weeks of feeding,sarcopenic obesity model mice were screened by body mass and behavior assessments.The sarcopenia obesity mice were then divided into the sedentary and exercise groups.Mice in the exercise group were subjected to treadmill training on the basis of a high-fat diet.After 8 weeks of intervention,body mass,lipid metabolism,muscle volume of calf muscle group of the hind limb,skeletal muscle morphology,and expressions of energy metabolic pathway-related genes were detected.RESULTS AND CONCLUSION:Compared with the normal control group,the levels of triglycerides,total cholesterol,and free fatty acid in the sedentary group were significantly increased,along with significantly increased lipid droplets in skeletal muscle(P<0.05).Compared with the sedentary group,all of the above indicators in the exercise group showed a significant decreasing trend(P<0.05).Compared with the normal control group,grip strength and fatigue latency time,muscle volume,and fiber cross-sectional area were significantly decreased in the sedentary group,but the mRNA expression of Atrogin-1 and Murf-1 was elevated(P<0.05).Compared with the sedentary group,grip strength and fatigue latency time,muscle volume,and fiber cross-sectional area were significantly improved in the exercise group(P<0.05),while the mRNA expression of Atrogin-1 and Murf-1 was reduced(P<0.05).Compared with the normal control group,the mRNA expression of intramuscular transcription factors Pparαand Pgc-1αwa

关 键 词：肌少性肥胖 跑台运动 有氧运动 脂质代谢 骨骼肌 脂肪酸β氧化 脂肪酸代谢 

分 类 号：R459.9[医药卫生—治疗学] R319[医药卫生—临床医学] G804.2[文化科学—运动人体科学]