1,8-桉叶油素干预大鼠实验性牙周炎模型的炎症反应  

Effects of 1,8-cineole on inflammatory response in a rat model of experimental periodontitis

作　　者：何丽 任潞 江小茜 刘旭倩 黎春晖 He Li;Ren Lu;Jiang Xiaoxi;Liu Xuqian;Li Chunhui(Department of Periodontics&Oral Mucosal Diseases,The Affiliated Stomatological Hospital,Southwest Medical University,Luzhou 646000,Sichuan Province,China;Luzhou Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,Institute of Stomatology,The Affiliated Stomatological Hospital,Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学附属口腔医院牙周黏膜病科,四川省泸州市646000 [2]西南医科大学附属口腔医院口腔医学研究所,口颌面修复重建和再生泸州市重点实验室,四川省泸州市646000

出　　处：《中国组织工程研究》2025年第17期3605-3613,共9页Chinese Journal of Tissue Engineering Research

基　　金：口腔疾病防治全国重点实验室开放课题项目(SKLOD2024OF04),项目负责人:刘旭倩;四川省医学科研课题计划项目(S23043),项目负责人:刘旭倩;泸州市科学技术和人才工作局科技创新领军人才计划项目(2023RCX171),项目负责人:刘旭倩;西南医科大学附属口腔医院“瑞泰育苗基金”科研培育计划项目(2022RT07),项目负责人:何丽。

摘　　要：背景:研究表明,天然植物精油提取物1,8-桉叶油素具有抗炎、抗氧化、抗菌、抗肿瘤等多种药理作用,在多种疾病中表现出抗炎作用。目的:探讨1,8-桉叶油素对大鼠实验性牙周炎模型炎症反应的影响。方法:按照完全随机数字表法将30只SD大鼠随机分为正常对照组、牙周炎对照组和1,8-桉叶油素组,每组10只。牙周炎对照组和1,8-桉叶油素组利用正畸钢丝结扎法构建实验性牙周炎模型。造模8周后,正常对照组和牙周炎对照组大鼠颊、腭侧牙周袋内均注射生理盐水,1,8-桉叶油素组大鼠颊、腭侧牙周袋内注射1,8-桉叶油素溶液,2次/d,连续给药4周。给药结束后,进行牙周临床指标检测、牙周组织学评估、血清炎症因子水平检测及牙龈组织中炎症因子m RNA与蛋白表达检测。结果与结论:①与正常对照组比较,牙周炎对照组大鼠牙龈出血指数、牙周探诊深度增加(P<0.05),血清中白细胞介素1β、肿瘤坏死因子α、白细胞介素6水平升高(P<0.05),血清中白细胞介素10水平降低(P<0.05),牙龈组织中白细胞介素1β、肿瘤坏死因子α、白细胞介素6mRNA与蛋白表达均升高(P<0.05),牙龈组织中白细胞介素10 mRNA与蛋白表达均降低(P<0.05);苏木精-伊红染色结果显示,牙周炎对照组大鼠牙周组织炎症明显。②与牙周炎对照组比较,1,8-桉叶油素组大鼠牙龈出血指数、牙周探诊深度减少(P<0.05),血清中白细胞介素1β、肿瘤坏死因子α、白细胞介素6水平降低(P<0.05),血清中白细胞介素10水平升高(P<0.05),牙龈组织中白细胞介素1β、肿瘤坏死因子α、白细胞介素6 mRNA与蛋白表达均降低(P<0.05),牙龈组织中白细胞介素10 mRNA与蛋白表达均升高(P<0.05);苏木精-伊红染色结果显示,1,8-桉叶油素组大鼠牙周组织炎症明显减轻。结果表明,1,8-桉叶油素治疗可减轻大鼠实验性牙周炎模型的炎症反应。BACKGROUND:Previous studies have shown that 1,8-cineole has anti-inflammatory,antioxidation,antibacterial and anti-tumor effects.It has good antiinflammatory effects in many diseases.OBJECTIVE:To investigate the effect of 1,8-cineole on inflammatory response in a rat model of experimental periodontitis.METHODS:Thirty Sprague-Dawley rats were assigned into normal control group,periodontitis control group and 1,8-cineole group with ten rats in each group according to the completely randomized digital table method.Except for the normal control group,rats in the other groups were induced into experimental periodontitis.The periodontitis model was constructed by the orthodontic ligature wire method.Eight weeks after modeling,in the 1,8-cineole group,1,8-cineole was placed into periodontal pockets,twice per day for 4 weeks.In the normal control group and the periodontitis control group,the same amount of normal saline was placed into periodontal pockets,twice per day for 4 weeks.After administration,general observation and periodontal clinical indicators were performed.Hematoxylin-eosin staining was used for periodontal histological evaluation.The expressions of inflammatory factors in the serum and gingiva at mRNA and protein levels were detected.RESULTS AND CONCLUSION:(1)Compared with the normal control group,rats in the periodontitis control group showed increased gingival bleeding index and periodontal probing depth(P<0.05),increased serum levels of interleukin 1β,tumor necrosis factorα,and interleukin 6(P<0.05),decreased serum level of interleukin 10(P<0.05),increased mRNA and protein levels of interleukin 1β,tumor necrosis factorα,and interleukin 6 in gingival tissue(P<0.05),and decreased mRNA and protein level of interleukin 10 in gingival tissue(P<0.05).Hematoxylin-eosin staining of periodontal tissues showed that compared with the normal control group,periodontal inflammation was obvious in the periodontitis control group.(2)Compared with the periodontitis control group,rats in the 1,8-cineole group showed

关 键 词：1 8-桉叶油素 植物精油 牙周炎 动物模型 牙周组织 炎症反应 白细胞介素1Β 肿瘤坏死因子α 白细胞介素6 白细胞介素10 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R313[医药卫生—临床医学] R781.4