糖尿病肾病模型:动物模型、二维细胞模拟及三维类器官模型  

Diabetic nephropathy model:animal model,two-dimensional cell simulation and three-dimensional organoid model

作　　者：钱足平 陈勇[2] 冉燕[2] 达静静[2] 查艳 Qian Zuping;Chen Yong;Ran Yan;Da Jingjing;Zha Yan(Zunyi Medical University,Zunyi 563000,Guizhou Province,China;Department of Nephrology,Guizhou Provincial People’s Hospital,Guiyang 550002,Guizhou Province,China)

机构地区：[1]遵义医科大学,贵州省遵义市563000 [2]贵州省人民医院肾内科,贵州省贵阳市550002

出　　处：《中国组织工程研究》2025年第17期3632-3640,共9页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(82360148),项目负责人:查艳;贵州省科技厅(黔科合成果[2023]重大010),项目负责人:查艳;贵州省高层次创新人才项目(黔科合平台人才[2018]5636-2),项目负责人:查艳;贵州省科技计划项目(黔科合平台人才[2020]2201号),项目负责人:查艳;贵州省科技计划项目(黔科合基础-ZK[2021]一般381),项目负责人:冉燕;贵州省科技计划项目(黔科合基础-ZK[2023]一般219),项目负责人:查艳;贵州省人民医院人才基金([2023]-8),项目负责人:陈勇。

摘　　要：背景:近几年来,人类多能干细胞衍生的肾类器官及糖尿病肾病啮齿动物模型取得了一定的进展。然而,由于糖尿病肾病发病受环境因素及遗传因素共同作用,发病机制复杂,并且对于此类患者的临床治疗方法需要因人而异。因此,需要开发更灵活和综合的方法,从而发现强有力的临床前证据,以支持对糖尿病肾病患者进行更有针对性的干预。目的:从动物模型、二维细胞培养模拟及三维类器官模型等方面综述了糖尿病肾病模型的研究进展,为进一步的研究提供线索和思路。方法:以“糖尿病,糖尿病肾病,糖尿病肾病模型,糖尿病肾病动物模型,类器官,肾脏类器官,糖尿病肾病细胞模型,糖尿病肾病病证结合动物模型”为中文检索词,“Diabetes,diabetic nephropathy,diabetic kidney diseases,diabetic nephropathy models,diabetic nephropathy animal models,organoids,diabetic and organoids,diabetic and kidney organoids,kidney organoids,diabetic nephropathy cell models,diabetic nephropathy syndrome combined animal models”为英文检索词,检索中国知网及PubMed数据库,最终纳入101篇文献进行综述分析。结果与结论:糖尿病肾病体内模型和体外模型是深入研究糖尿病肾病发病机制的有力工具。动物模型可以观察到多系统之间的相互作用;二维细胞培养操作简便且成本较低;新兴的肾类器官填补了二维和整体水平之间的空白,无种属差异且一定程度模拟了人肾脏的复杂性。随着类器官技术的不断发展,肾类器官有望为探索糖尿病肾病的发病机制、病理生理过程和药物筛选提供一个新的视角。BACKGROUND:In recent years,human pluripotent stem cell derived kidney organoids and rodent models of diabetic kidney disease have also made some progress.However,because the pathogenesis of diabetic kidney disease is affected by environmental factors and genetic factors,the pathogenesis is complex,and the clinical treatment for diabetic kidney disease patients needs to vary from person to person.Therefore,more flexible and integrated approaches need to be developed,leading to the discovery of strong preclinical evidence to support more targeted interventions in patients with diabetic kidney disease.OBJECTIVE:To reviewthe research progress of diabetic kidney disease model from the aspects of diabetic kidney disease animal model,two-dimensional cell culture simulation diabetic kidney disease model,and three-dimensional organoid diabetic kidney disease model,to provide clues and ideas for further research.METHODS:China National Knowledge Network and PubMed databases were searched with“diabetes,diabetic nephropathy,diabetic kidney diseases,diabetic nephropathy models,diabetic nephropathy animal models,organoids,diabetic and organoids,diabetic and kidney organoids,kidney organoids,diabetic nephropathy cell models,diabetic nephropathy syndrome combined animal models”as Chinese and English search terms.Totally 101 articles were finally included for review and analysis.RESULTS AND CONCLUSION:Both in vivo and in vitro models of diabetic kidney disease are powerful tools to further study the pathogenesis of diabetic kidney disease.The interactions between multiple systems can be observed in animal models.Two-dimensional cell culture is easy to operate and low cost.The emerging kidney organoids fill the gap between two-dimensional and global levels,without species differences,and simulate the complexity of human kidney to a certain extent.With the continuous development of organoid technology,kidney organoids are expected to provide a new perspective for exploring the pathogenesis,pathophysiology,and drug screening of d

关 键 词：糖尿病肾病 肾脏类器官 糖尿病肾病类器官 细胞模型 动物模型 中医病证结合糖尿病肾病模型 工程化组织构建 

分 类 号：R496[医药卫生—康复医学] R363[医药卫生—临床医学] R587.2