永乐环礁表层海水细菌群落多样性及功能预测  

Diversity and Functional Prediction of Bacterial Communities in Surface Seawater of Yongle Atoll

作　　者：舒艾梅 宫春光[1] 黄惠琴[2] 胡永华[2,3] 莫坤联 SHU Aimei;GONG Chunguang;HUANG Huiqin;HU Yonghua;MO Kunlian(College of Oceanography,Agriculture University of Hebei,Qinhuangdao 066003,China;Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou 571101,China;Zhanjiang Experimental Station,CATAS,Zhanjiang 524013,China)

机构地区：[1]河北农业大学海洋学院,河北秦皇岛066003 [2]中国热带农业科学院热带生物技术研究所,海南海口571101 [3]中国热带农业科学院湛江实验站,广东湛江524013

出　　处：《海洋科学进展》2024年第4期725-737,共13页Advances in Marine Science

基　　金：海南省自然科学基金青年基金项目(323QN278);农业农村部财政专项(NFZX2021)。

摘　　要：为探究永乐环礁表层海水环境中细菌群落结构组成以及差异性,采集了6个岛礁的表层海水样品,利用高通量宏条形码测序技术分析了不同岛礁海水细菌的群落结构组成差异,并探索了环境因子对细菌群落结构的影响,同时对菌群功能基因进行了预测分析。共获得优化序列891 892条,聚类为7 499个操作分类单元(Operational Taxonomic Units, OTUs),隶属于49个门、144个纲、392个目、663个科和1 274个属。优势细菌类群主要包括变形菌门(Proteobacteria)、蓝细菌门(Cyanobacteriota)、拟杆菌门(Bacteroidota)、放线菌门(Actinobacteriota)、髌骨细菌门(Patescibacteria)和厚壁菌门(Firmicutes)。通过冗余分析发现主要的环境影响因子为电导率、pH值和温度。PICRUSt功能预测结果显示不同岛礁中细菌主要涉及6个生物代谢通路及46个子功能,共得到399个三级通路相关功能基因,其中代谢途径和次生代谢产物等方面的功能基因较为丰富。本文初步揭示了永乐环礁6个岛屿表层海水微生物群落组成与基因功能预测,可为研究永乐群岛岛礁生态及环境保护提供一定的参考,并为后续进一步的微生物资源调查和开发利用提供基础研究数据。To investigate the bacterial community structure,composition and diversity in surface seawater of Yongle atoll,Illumina MiSeq sequencing was used to analyze the difference in composition of the bacterial communities at six islands and reefs.The main environmental impact factors affecting bacteria community structure were explored and the functional genes of bacteria communty were also predicted.A total of 891892 high-quality sequences clustering 7499 optimized OTUs were obtained.These OTUs were subordinated to 49 phyla,144 classes,392 orders,663 families,and 1274 genera.The bacteria communities were dominated by Proteobacteria,Cyanobacteria,Bacteroidetes,Actinobacteria,Patescibacteria and Firmicutes.Redundancy analysis revealed that the conductivity,pH and temperature were the main environmental factors affecting the microbial assemblages.The results of PICRUSt analysis showed that 6 biological metabolic pathways and 46 subfunctionalities were predicted;a total of 399 functional genes at level 3 pathways were obtained,of which the most abundant functional genes were related to metabolic pathways and the biosynthesis of secondary metabolites.This study analyzes the bacterial community compositions in surface seawater and predicts their gene functions at six islands and reefs of Yongle atoll,providing a basic data for further investigation and development of microbial resources.

关 键 词：永乐环礁 细菌多样性 环境因子 功能预测 

分 类 号：Q938[生物学—微生物学]