群体感应对藻际细菌Sulfitobacter pseudonitzschiae H46生理活性调控的研究  

Study on the Biological Activities of Phycospheric Bacterium Sulfitobacter Pseudonitzschiae H46 Regulated by Quorum Sensing

作　　者：迟旭辉 杨淋 张彦超 胡天翼 王帅 郑立 CHI Xuhui;YANG Lin;ZHANG Yanchao;HU Tianyi;WANG Shuai;ZHENG Li(Qingdao Key Laboratory of Analytical Technology Development and Offshore Eco-Environment Conservation,First Institute of Oceanography,MNR,Qingdao 266061,China;Laboratory for Marine Ecology and Environmental Science,Laoshan Laboratory,Qingdao 266237,China)

机构地区：[1]自然资源部第一海洋研究所青岛市现代分析与近海生态环境安全保障重点实验室,山东青岛266061 [2]崂山实验室海洋生态与环境科学功能实验室,山东青岛266237

出　　处：《海洋科学进展》2024年第4期738-752,共15页Advances in Marine Science

基　　金：中央级公益性科研院所基本科研业务费专项资金资助项目(2021Q10);国家自然科学基金项目(41776176和4180620);泰山产业领军人才工程专项经费项目(tscx202306084);国家留学基金项目(2022)。

摘　　要：针对海洋细菌群体感应调控功能的热点问题,研究了藻际细菌Sulfitobacter pseudonitzschiae H46的群体感应系统对菌株H46的生态功能和抑藻活性的影响,探讨了基于群体感应介导的藻菌相互作用关系。通过添加不同浓度群体感应抑制剂(β-环糊精),分析了亚硫杆菌H46成膜性、DMSP降解能力、抑藻活性的变化,了解了亚硫杆菌H46群体感应系统潜在的调控功能。结果表明,β-环糊精在一定程度上淬灭了亚硫杆菌H46的群体感应信号分子AHLs的诱导活性,导致亚硫杆菌H46的成膜能力、DMSP降解能力和抑藻活性受到抑制。本文以群体感应调节细菌生态学功能为切入点,探讨了藻际细菌对微藻生长的抑制作用,为藻菌互作机制研究提供新的视角。This paper focused on the hot issue of quorum sensing regulatory function of marine bacteria.The influence of the quorum sensing system of the phycospheric Sulfitobacter pseudonitzschiae H46 on its ecological function and algae inhibition was studied and algal-bacterial interaction modulated by quorum sensing was explored.H46 was co-cultured with different concentrations of quorum sensing inhibitor(β-cyclodextrin).Then the functions of biofilm formation,DMSP degradation and algicidal activity of H46 were analyzed.And the potential regulation function of H46 quorum sensing system was confirmed.The results showed that the induced activity of the quorum sensing signaling molecule AHLs of H46 was partially quenched byβ-CD.And then the biological ability of membrane forming,DMSP degradation and alga-inhibit of H46 were inhibited.In this paper,we explore the inhibitory effect of bacteria on the growth of microalgae,which provides a new perspective for the study of mechanism of algal-bacterial interaction.

关 键 词：藻菌互作 藻际环境 亚硫杆菌 群体感应 抑藻活性 

分 类 号：Q93-3[生物学—微生物学]