基于Box-Behnken设计-响应面法优化槲寄生提取工艺研究  

Research on the Optimization of Mistletoe Extraction Process Based on Box-Behnken Design-Response Surface Method

作　　者：张奡锴 李文杰[1] 董扬扬 褚寒冰 董学海 赵丽艳 张万明[1] 张元元 ZHANG Ao-kai;LI Wen-jie;DONG Yang-yang;CHU Han-bing;DONG Xue-hai;ZHAO Li-yan;ZHANG Wan-ming;ZHANG Yuan-yuan(College of Pharmacy,Hebei North University,Hebei Key Laboratory of Neuropharmacology,Zhangjiakou,075000,China)

机构地区：[1]河北北方学院药学院,河北省神经药理学重点实验室,张家口075000

出　　处：《神经药理学报》2024年第4期1-8,17,共9页Acta Neuropharmacologica

基　　金：河北省大学生创新创业训练计划项目(No.202310092010);河北省自然科学基金项目(No.H2023405003);河北省教育厅高校基本科研业务费(No.JYT2020002);河北省卫生健康委科研基金项目(No.20180812)。

摘　　要：目的:采用Box-Behnken设计-响应面法,以两种活性成分综合含量为评价指标,优化槲寄生提取工艺。方法:以紫丁香苷、高圣草素-7-O-β-D-葡萄糖苷含量为评价指标,在单因素基础上,采用Box-Behnken设计-响应面法对槲寄生提取工艺的乙醇体积分数、提取时间、液料比进行优化。采用超高效液相色谱(ultra performance liquid chromatography,UPLC)对14个产地的槲寄生中的两种活性成分进行含量测定。结果:槲寄生最佳提取工艺为:71%乙醇-水溶液,液料比25:1,提取时间67 min。通过最佳提取工艺测得,紫丁香苷与高圣草素-7-O-β-D-葡萄糖苷权重占比为7:3时,综合含量平均值为571.7μg·g^(-1)。紫丁香苷、高圣草素-7-O-β-D-葡萄糖苷的含量范围分别为128.7~662.8μg·g^(-1)、77.24~801.8μg·g^(-1)。不同产地槲寄生中紫丁香苷、高圣草素-7-O-β-D-葡萄糖苷含量不同,其中四川产地槲寄生中高圣草素-7-O-β-D-葡萄糖苷含量相对较高为0.84 mg·g^(-1),安徽产地槲寄生中紫丁香苷含量相对较高为0.47 mg·g^(-1)。结论:本研究所建立的方法灵敏度高、分离效果好、结果稳定可靠,可为槲寄生的有效成分提取、质量评价及资源开发利用提供科学依据。Objective:To optimize the extraction process of Mistletoe by using the Box-Behnken design-response surface method,and the comprehensive content of the two active ingredients as the evaluation index.Methods:The ethanol volume fraction,extraction time and solid-liquid ratio of Mistletoe extraction process were optimized by using the Box-Behnken design-response surface method on the basis of single factor.Ultra performance liquid chromatography(UPLC)was used to determine the content of two active components in Mistletoe from 14 origins.Results:The optimal extraction process of Mistletoe was 71%ethanol-aqueous solution,liquid-solid ratio 25:1,and extraction time 67 min.The optimal extraction process showed that the average comprehensive content was 571.7μg·g^(-1) when the weight ratio of Syringin to homoeriodictyol-7-O-β-D-glucoside was 7:3.The contents of Syringin and-1 homoeriodictyol-7-O-β-D-glucoside ranged from 128.7~662.8μg·g and 77.24~801.8μg·g^(-1).The contents of Syringin and homoeriodictyol-7-O-β-D-glucoside in Mistletoe from different origins were different,among which the content of homoeriodictyol-7-O-β-D-glucoside in Mistletoe from Sichuan was relatively high,which was 0.84 mg·g^(-1),and the content of Syringin from Mistletoe from Anhui was relatively high at 0.47 mg·g^(-1).Conclusion:The method established in this study has high sensitivity,good separation effect,stable and reliable results,which can provide a scientific basis for the extraction of active ingredients,quality evaluation and resource development and utilization of Mistletoe.

关 键 词：槲寄生 响应面法 含量测定 紫丁香苷 高圣草素-7-O-β-D-葡萄糖苷 

分 类 号：R286[医药卫生—中药学]