超声微泡辅助治疗对大鼠脑缺血再灌注损伤的治疗效果探讨  

Exploration of the Therapeutic Effect of Ultrasound Microbubble-Assisted Therapy on Cerebral Ischemia-Reperfusion Injury in Rats

作　　者：马洁华[1] 王慕通 郭帆 刘旭 王悦瑶 赵宇 张辉[1] MA Jie-hua;WANG Mu-tong;GUO Fan;LIU Xu;WANG Yue-yao;ZHAO Yu;ZHANG Hui(School of Basic Medicine,Hebei North University,Zhangjiakou,075000,China;The First Clinical Medical College,Hebei North University,Zhangjiakou,075000,China)

机构地区：[1]河北北方学院基础医学院,张家口075000 [2]河北北方学院第一临床医学院,张家口075000

出　　处：《神经药理学报》2024年第4期34-37,48,共5页Acta Neuropharmacologica

基　　金：河北北方学院校级青年基金项目(No.QN2018014);河北省卫生计生委医学科学研究项目(No.20180809);河北北方学院大学生创新创业训练计划项目(No.XJ202216)。

摘　　要：目的:探讨超声微泡辅助治疗对大鼠脑缺血再灌注损伤的治疗效果。方法:采用线栓法制备大鼠脑缺血再灌注模型,取造模成功的40只大鼠随机分为微泡组(超声辐照+微泡+溶栓)和对照组(超声辐照+溶栓)各20例。2组大鼠均以1次·d^(-1)的频率接受治疗,持续治疗15 d。溶栓治疗结束后,比较2组血栓清除评分、神经损伤严重情况、脑组织含水率。结果:分组后3、6 h微泡组血栓清除评分高于对照组(P<0.05);分组后10、15 d微泡组改良神经功能缺损评分(modified neurological severity scores,mNSS)评分均低于对照组(P<0.05);分组后8 d,微泡组脑组织含水率低于对照组(P<0.05)。结论:超声联合微泡用于脑缺血再灌注大鼠,有助于血栓的清除,能够减轻神经损伤及脑组织含水率。Objective:To explore the therapeutic effect of ultrasound microbubble-assisted therapy on cerebral ischemia-reperfusion injury in rats.Methods:A rat cerebral ischemia-reperfusion model was prepared by the wire thrombus method.40 successfully modeled rats were randomly divided into a microbubble group(ultrasound irradiation+microbubble+thromb olysis)and a control group(ultrasound irradiation+thrombolysis),with 20 cases in each group.Both groups of rats received treatment at a frequency of 1 times·d^(-1) for 15 days.At the end of thrombolysis treatment,thrombus clearance scores,severity of neurological damage,and water content of the brain tissue were compared between the two groups.Results:Thrombus clearance score in the microbubble group was higher than that in the control group at 3 and 6 h after grouping(P<0.05).mNSS scores in the microbubble group were lower than that in the control group on the 10th and 15th day after grouping(P<0.05).The water content of brain tissue in the microbubble group was lower than that in the control group on the 8th day after grouping(P<0.05).Conclusion:Ultrasound combined with microbubbles for cerebral ischemia-reperfusion rats contributes to thrombus clearance and is able to attenuate neurological damage and brain tissue water content.

关 键 词：超声微泡 脑缺血 再灌注 动物实验 

分 类 号：R964[医药卫生—药理学]