3种石斛种子无菌播种快繁技术  

Rapid Propagation Technique of 3 Kinds of Dendrobium Seeds

作　　者：令狐克念 杨焱冰 颜凤霞 田凡 许志高 代丽 王莲辉 LINGHU Ke-nian;YANG Yan-bing;YAN Feng-xia(Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China,Guizhou Academy of Forestry,Guiyang,Guizhou 550005)

机构地区：[1]贵州省林业科学研究院西南喀斯特山地生物多样性保护国家林业和草原局重点实验室,贵州贵阳550005

出　　处：《安徽农业科学》2024年第20期155-159,共5页Journal of Anhui Agricultural Sciences

基　　金：贵州省林业局(黔林科合〔2021〕02号);贵州省林业局(黔林科合J字〔2022〕11号);贵州省林业局(黔林科合〔2019〕02号);贵州石斛种质资源库建设项目(黔石科合2019008)。

摘　　要：为了解鼓槌石斛、细茎石斛和球花石斛种子无菌播种快繁技术,以3种石斛种子为研究对象,探讨不同培养基、激素种类以及浓度配比对3种石斛种子无菌萌发、原球茎增殖和种苗壮苗生根的影响。结果表明:3种石斛种子的萌发率最高均在1/2MS基础培养基和添加10%马铃薯的1/2MS培养基上;鼓槌石斛和球花石斛原球茎增殖培养基以1/2MS+10%马铃薯+0.5 mg/L 6-BA+0.2 mg/L NAA最佳,细茎石斛原球茎增殖培养基以1/2MS+10%马铃薯+1.0 mg/L 6-BA+0.2 mg/L NAA最佳;鼓槌石斛生根壮苗培养基以1/2MS+0.10 mg/L IBA+10%马铃薯+2.0%蔗糖+0.6%琼脂最佳,球花石斛和细茎石斛的生根壮苗培养基以1/2MS+0.50 mg/L IBA+10%马铃薯+2.0%蔗糖+0.6%琼脂最佳。To understand the Dendrbium chrysotoxum,Dendrobium moniliforme and Dendrobium thyrsiflorum seed sterile plant rapid propagation techniques,in this paper,three kinds of Dendrobium seeds as the research object,discusses different types of medium,hormone and concentration ratio of 3 kinds of Dendrobium sterile seed germination,protocorm proliferation and seedling seedling rooting effect.The results showed that the highest germination rate of the 3 Dendrobium seeds was on 1/2MS base culture and 1/2MS medium with 10%potato.Dendrobium chrysotoxum and Dendrobium thyrsiflorum were optimized with 1/2MS+10%potato+0.50 mg/L 6-BA+0.2 mg/L NAA,while Dendrobium moniliforme was optimized with 1/2MS+10%potato+1.0 mg/L 6-BA+0.2 mg/L NAA.The best rooting and seedling medium of Dendrobium chrysotoxum moniliforme was 1/2MS+0.10 mg/L IBA+10%potato+2.0%sucrose+0.6%AGAR,and the best rooting and seedling medium of Dendrobium thyrsiflorum and Dendrobium moniliforme was 1/2MS+0.50 mg/L IBA+10%potato+2.0%sucrose+0.6%AGAR.

关 键 词：石斛属 无菌萌发 原球茎增殖 壮苗生根 

分 类 号：S567.23[农业科学—中草药栽培]