四川省道孚县牦牛源蜱携带巴尔通体、无形体和立克次体的检测与进化分析  

Detection and Phylogenetic Analysis of Bartonella,Anaplasma and Spotted Fever Group Rickettsia in Ticks from Yaks in Daofu County of Sichuan Province

作　　者：唐天才 蓝岚 高文武 陈天祥 肖晨冬 郝力力[5] 罗勇 陈和强 杨兴康 潘瑶 尹念春 TANG Tiancai;LAN Lan;GAO Wenwu;CHEN Tianxiang;XIAO Chendong;HAO Lili;LUO Yong;CHEN Heqiang;YANG Xingkang;PAN Yao;YIN Nianchun(Yibin University,Yibin Sichuan 644000,China;Animal Husbandry Research Institute of Ganzi Tibetan Autonomous Prefecture,Ganzi Sichuan 626000,China;Ruoergai County Bureau of Science,Technology,Agriculture,and Animal Husbandry,Ruoergai Sichuan624000,China;Center for Animal Disease Control and Prevention of Xiangcheng County,Xiangcheng Sichuan 627850,China;Southwest Minzu University,Chengdu 610041,China;Center for Animal Disease Control and Prevention of Seda County,Seda Sichuan 624300,China;Center for Animal Disease Control and Prevention of Ganzi Prefecture,Ganzi Sichuan 626000,China;Center for Animal Disease Control and Prevention of Jiulong County,Jiulong Sichuan 616200,China;Suining Agriculture and Rural Bureau,Suining Sichuan 629000,China)

机构地区：[1]宜宾学院,四川宜宾644000 [2]甘孜藏族自治州畜牧业科学研究所,四川甘孜州626000 [3]若尔盖县科学技术和农业畜牧局,四川若尔盖624000 [4]乡城县动物疫病预防控制中心,四川乡城县627850 [5]西南民族大学,成都610041 [6]色达县动物疫病预防控制中心,四川色达624300 [7]甘孜州动物疫病预防控制中心,四川甘孜州626000 [8]九龙县动物疫病预防控制中心,四川九龙616200 [9]遂宁市农业农村局,四川遂宁629000

出　　处：《西北农业学报》2024年第10期1807-1818,共12页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：宜宾学院教师培育项目(412-2021PY066)。

摘　　要：为了解四川省道孚县牦牛体表蜱的种类及其携带病原情况,采集四川省道孚县七美乡、龙灯乡、瓦日乡和八美镇的家养牦牛体表寄生蜱,先对其进行形态学和分子生物学鉴定,然后采用巢氏PCR扩增蜱类样本中巴尔通体gltA和rpoB、无形体16S rRNA和斑点热群立克次体ompA基因,再对阳性产物进行测序和比对,并构建进化树。结果表明,共采集蜱虫1280只,经鉴定,隶属于1科4属4种,分别为微小扇头蜱、卵形硬蜱、森林革蜱和尼泊尔血蜱。PCR检测结果显示,巴尔通体、无形体和斑点热群立克次体的检出率分别为18.67%(239/1280)、31.02%(397/1280)和43.75%(560/1280)。在道孚县4个采样地区的蜱中,八美镇巴尔通体检出率最高,为33.61%(80/238);七美乡无形体检出率最高,为48.71%(170/349);瓦日乡斑点热群立克次体检出率最高,为57.57%(213/370)。在4种蜱中,微小扇头蜱的巴尔通体和斑点热群立克次体检出率最高,分别为28.78%(136/473)和62.58%(296/473);尼泊尔血蜱的无形体检出率最高,为38.25%(83/217)。另外,有248只蜱存在2种或3种病原的复合感染,复合感染率为19.38%(248/1280)。遗传进化分析显示,巴尔通体、无形体和斑点热群立克次体均只有1个种,分别是B.melophagi、A.marginale和Candidatus R.longicornii。表明道孚县存在微小扇头蜱、卵形硬蜱、森林革蜱和尼泊尔血蜱,蜱中广泛存在B.melophagi、A.marginale和Candidatus R.longicornii的感染,同时存在病原复合感染,因此需要重点加强该地区蜱媒传染病的监测和预防。To investigate tick species and tick-borne pathogens from yaks in Daofu County,Sichuan Province,the ticks were collected from domesticated yaks in Qimei,Wari,Longdeng and Bamei,Daofu County,Sichuan Province.The ticks were then classified by morphological and molecular identification.The partial sequences of gltA and rpoB gene of Bartonella,the 16S rRNA gene of Anplasma and ompA gene of Spotted Fever Group Rickettsia(SFGR)were amplified using nested PCR,respectively.The positive products were sequenced and compared through the NCBI database.Phylogenetic trees were constructed using the adjoining method.The results showed that a total of 1280 ticks were collected and identified,belonging to 4 species,4 genera and 1 family.The identified tick species were Rhipicephalus microplus,Ixodes ovatus,Dermacentor silvarum and Haemaphysalis nepalensis.Based on the PCR results,the detection rates of Bartonella,Anaplasma and SFGR in these ticks were 18.67%(239/1280),31.02%(397/1280)and 43.75%(560/1280),respectively.Among the four sampling areas,the highest detection rates of Bartonella,Anaplasma,and SFGR were observed in ticks collected from Bamei(36.1%,80/238),Qimei(48.71%,170/349),and Wari(57.57%,213/370),respectively.Among the four tick species,R.microplus exhibited the highest infection rates of Bartonella(28.78%,136/473)and SFGR(62.58%,296/473),while H.nepalensis had the highest detection rate of Anaplasma(38.25%,83/217).In addition,248 ticks were found to be infected with two or three pathogens,resulting in a co-infection rate of 19.38%(248/1280).Phylogenetic analysis revealed that Bartonella,Anaplasma and SFGR each had only one species,which were B.melophagi,A.marginale and Candidatus R.longicornii,respectively.Four tick species,R.microplus,I.ovatus,D.silvaru m and H.nepalensis,were found in Daofu County.B.melophagi,A.marginale and Candidatus R.longicornii were highly prevalent in the ticks.Meanwhile,co-infection of at least two pathogens was observed in 19.38%ticks.The results here indicate that it is necessary to stren

关 键 词：牦牛 蜱 巴尔通体 无形体 斑点热群立克次体 

分 类 号：S858.23[农业科学—临床兽医学]