两种藜麦幼苗对氮铁营养的生理生化响应  

Response of Physiological and Biochemical Traits in Seedlings of Two Dominant Quinoa Cultivars to Nitrogen and Iron Nutrition

作　　者：白丽丽 胡华伟 周华坤 王世雄[1,4] 王文颖 BAI Lili;HU Huawei;ZHOU Huakun;WANG Shixiong;WANG Wenying(Qinghai Normal University,Xining 810016,China;Xinjiang Acadeny of Forestry Science,Urumqi 830046,China;Academy of Plateau Science and Sustainability,Xining 810016,China;Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;Qinghai Provincial Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization in Qinghai Tibetan Plateau,Xining 810016,China)

机构地区：[1]青海师范大学,西宁810013 [2]新疆林业科学院,乌鲁木齐830046 [3]高原科学与可持续发展研究院,西宁810016 [4]中国科学院西北高原生物研究所,西宁810008 [5]青海省青藏高原生物多样性形成机制与综合利用重点实验室,西宁810016

出　　处：《西北农业学报》2024年第10期1839-1848,共10页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：2021年第一批中央林草生态保护恢复资金自然资源调查监测项目(QHXH-2021-017);青海省2021年度第一批中央引导地方科技发展专项资金(2021ZY002)。

摘　　要：本研究选取2种优势藜麦品种(红藜和黑藜),分析2个氮水平(N1,4 mmol·L^(-1);N2,12 mmol·L^(-1))和3个铁水平(Fe1,10μmol·L^(-1);Fe2,100μmol·L^(-1)和Fe3,300μmol·L^(-1))组合下的藜麦幼苗生理生化反应,旨在为滇中苗期藜麦合理补充氮、铁营养提供理论依据。结果表明:(1)红藜麦单株鲜质量大于黑藜麦。叶绿素含量在两藜麦中均为,在N1供应下配施Fe2最高,在N2供应下则配施Fe3最高。(2)MDA含量在两藜麦不同器官中均呈现叶>根。在同一铁供应下,两藜麦MDA含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性均表现为N2>N1。在两种氮供应下,红藜麦配施Fe1时叶片MDA含量较高;黑藜麦则在配施Fe2时最高。对于叶片各抗氧化酶活性,红藜麦在两种氮供应下均配施Fe3时SOD、APX活性最强;黑藜麦在N1供应下CAT、抗坏血酸过氧化物酶(APX)、SOD活性为Fe2>Fe3>Fe1,在N2供应下配施Fe3时CAT、APX活性最高。(3)在同一铁供应下,两藜麦脯氨酸含量、可溶性糖、可溶性蛋白均表现为N2>N1。在两种氮供养下,红藜麦在配施Fe3时根部脯氨酸含量累积较多;黑藜麦则在配施Fe2时最高。可溶性蛋白含量在两种氮供养下,红藜麦在配施Fe1时含量最高,黑藜麦则在Fe3时达到最大值。从以上研究结果得出:红藜麦为缺铁敏感型,黑藜麦则为耐逆型品种。在藜麦苗期合理配施氮铁营养,在N1供应下当配施Fe2较佳,在N2供应下当配施Fe3最宜。Two dominant quinoa cultivars(red and black)were selected for this study.Physiological and biochemical traits of quinoa seedlings were analyzed under the combined treatments of two nitrogen levels(N1:4 mmol·L^(-1);N2:12 mmol·L^(-1))and three iron levels(Fe1:10μmol·L^(-1);Fe2:100μmol·L^(-1) and Fe3:300μmol·L^(-1)).The analysis provides theoretical references for the appropriate supplementation of nitrogen and iron in the growth of different quinoa cultivars.The results showed that(1)plant biomass of red quinoa exceeded that of black quinoa.The total chlorophyll content in both quinoa cultivars was the highest in Fe2 under N1 supply conditions,but under N2 supply conditions,it was the highest in Fe3.(2)The content of malondialdehyde(MDA)in leaves was higher than that in roots.The MDA content,superoxide dismutase(SOD)and catalase(CAT)activities in both quinoa cultivars were higher under N2 than N1 conditions with the same iron supply.Under both N conditions,the MDA content of leaves was highest in Fe1 condition in red quinoa,while was highest in Fe2 condition in black quinoa.Regarding antioxidant enzyme activities in leaves,SOD and APX activities of red quinoa were the strongest when treated with Fe3 under both N conditions.For black quinoa,the activities of CAT,ascorbate peroxidase(APX)and SOD were highest under Fe2,followed by Fe3,and then Fe1 under N1 conditions.The activities of CAT and APX were the highest when Fe3 was combined with N2 supply.(3)The content of proline,soluble sugar,and soluble protein of two quinoa were higher under N2 than N1 conditions with the same iron supply.Under the nitrogen conditions,red quinoa accumulated more proline under Fe3 treatment,while black quinoa had the highest accumulation under Fe2.Under both two nitrogen conditions,red quinoa had the highest soluble protein content under Fe1 treatment,while black quinoa had the highest under Fe3 treatment.In conclusion,red quinoa demonstrates sensitivity and black quinoa exhibits resistance to iron deficiency.For optimal growth d

关 键 词：藜麦 幼苗 氮营养 铁营养 生理生化 

分 类 号：S519[农业科学—作物学]