不同处方的mRNA-LNP对细胞体外表达的影响  

Effect of Different Prescribed mRNA-LNP on Cell Expression in Vitro

作　　者：黄彬彬 彭保卫 Huang Binbin;Peng Baowei(Dali University,Dali 671000,China)

机构地区：[1]大理大学,云南大理671000

出　　处：《广东化工》2024年第19期17-19,共3页Guangdong Chemical Industry

摘　　要：核酸药物递送系统是一种有助于将核酸药物高效传递到细胞内并发挥作用的技术。尽管核酸药物具有高度的生物活性和特异性,但是其应用仍然面临着多种挑战。因此,对核酸递送系统处方的研究至关重要,为了探究不同处方的LNP对细胞的体外表达有影响,我们以GFP-mRNA作为测试基因,制备成不同处方的LNP,探索其作用机制,为此,我们制备了不同的LNP处方,选择具有高表达的293T细胞作为转染细胞,通过荧光倒置显微镜对其荧光强度进行检测,分析比较其转染效率,发现高比例的空载LNP会影响细胞的体外转染效率,为高质量mRNA药物制剂的研发打下了一定的基础。Nucleic acid drug delivery systems are technologies that facilitate the efficient delivery of nucleic acid drugs into cells,allowing them to exert their effects.Despite the high bioactivity and specificity of nucleic acid drugs,their applications still face various challenges.Therefore,research on the formulations of nucleic acid delivery systems is crucial.To investigate the impact of different formulations of lipid nanoparticles(LNPs)on in vitro expression in cells,we used GFP-mRNA as a test gene to prepare LNPs with different formulations and explore their mechanisms of action.In this study,we prepared various LNP formulations and chose 293T cells with high expression as the transfection cells.The fluorescence intensity was detected using a fluorescence inverted microscope to analyze and compare the transfection efficiency.We found that a high proportion of empty LNPs affects the in vitro transfection efficiency of cells,laying a foundation for the development of high-quality mRNA drug formulations.

关 键 词：脂质纳米颗粒 细胞转染 空载LNP 

分 类 号：TQ[化学工程]