MAdCAM抗体偶联脂质体的制备和结肠炎性组织结合研究  

Preparation of MAdCAM Antibody-conjugated Liposomes and Colonic Inflammatory Tissue Binding Studies

作　　者：邵榆 徐宇虹 Shao Yu;Xu Yuhong(College of Pharmacy,Dali University,Dali 671000,China)

机构地区：[1]大理大学药学院,云南大理671000

出　　处：《广东化工》2024年第19期82-84,共3页Guangdong Chemical Industry

摘　　要：本研究制备了靶向MAdCAM-1(Mucosal addressin cell adhesion molecule-1)的免疫脂质体(Immunoliposome),通过体外组织IHC实验验证其特异性结合能力,为治疗肠炎提供一个新的解决思路。本文首先把MECA-367蛋白(MadCAM蛋白抗体)通过SATA进行巯基引入后与DSPE-PEG-2K-MAL偶联,再在脂质体上进行装载。制备得到靶向MAdCAM免疫脂质体,以SDS-PAGE,HPLC和粒径为评价指标。同时建立DSS动物实验模型并进行IHC实验。SDS-PAGE条带可验证其蛋白偶联成功,制剂表征的结果显示,免疫脂质体平均粒径分别为87nm,且粒径分布均匀(多分散性指数,Polydispersity index,PDI≤0.1),HPLC验证其装载效率100%,BLI验证其亲合力不错,IHC结果阳性验证了其结合能力。最终成功制备了MAdCAM抗体偶联脂质体,且在体外实验成功验证其与结肠炎性组织特异性结合。证明此制备工艺方法具有可行性,可进行持续探索。In this study,an immunoliposome targeting MAdCAM-1(Mucosal addressin cell adhesion molecule-1)was prepared,and its specific binding ability was verified by in vitro tissue IHC experiments,so as to provide a new solution for the treatment of enteritis.In this paper,MECA-367 protein(MadCAM protein antibody)was first introduced by SATA for sulfhydryl group introduction,then conjugated to DSPE-PEG-2K-MAL,and then loaded on liposomes.Immunoliposomes targeting MAdCAM were prep ared,and SDS-PAGE,HPLC and particle size were used as evaluation indexes.the DSS animal experimental model was established and IHC experiments were performed.The SDS-PAGE bands were successfully conjugated,and the results of preparation characterization showed that the average particle size of immunoliposomes was 87 nm,and the particle size distribution was uniform(polydispersity index,PDI≤0.1),the loading efficiency was 100%verified by HPLC results,the affinity was good by BLI,and the binding capacity was verified by positive IHC results.Conclusion:In this paper,MAdCAM antibody-conjugated liposomes were successfully prepared,and its specific binding to colonic inflammatory tissues was successfully verified in vitro experiments.This preparation process method is proved to be feasible and can be continuously explored.

关 键 词：MAdCAM 脂质体 IBD 

分 类 号：R94[医药卫生—药剂学]