金毛耳草药材的质量标准研究  

Study on Quality Standard of Hedyotis chrysotricha

作　　者：崔田[1] 邓祖磊 Cui Tian;Deng Zulei(Bengbu Institute for Food and Drug Control,Bengbu 233000,China)

机构地区：[1]蚌埠市食品药品检验中心,安徽蚌埠233000

出　　处：《广东化工》2024年第20期171-173,共3页Guangdong Chemical Industry

基　　金：安徽省中药材标准研究课题(药监办许可函[2020]218号)。

摘　　要：目的:建立金毛耳草药材的质量标准。方法:采用显微鉴定、薄层色谱对金毛耳草进行定性鉴别;用中国药典法对金毛耳草的水分、总灰分、酸不溶性灰分和浸出物测定;采用HPLC法测定熊果酸和齐墩果酸含量,色谱柱为Agilent ZORBAX Eclipse XDB-C_(18);流动相:甲醇-0.2%醋酸溶液(88∶12);流速:0.8 mL/min;检测波长:210 nm;柱温:30℃。结果:显微特征明显;薄层斑点清晰,分离度好,专属性强;熊果酸和齐墩果酸分别在0.0376 mg/mL~0.9399 mg/mL、0.0074 mg/mL~0.1845 mg/mL范围内线性关系良好,平均加样回收率分别为98.61%、97.63%,RSD分别为1.07%、1.13%。测得药材样品总灰分为11.4%~21.4%,酸不溶性灰分为3.0%~11.9%,水分为8.4%~11.0%,含量为1.43 mg/g~3.41 mg/g。结论:该方法合理可行,可用于金毛耳草药材质量控制。Objective:To establish the quality standard for Hedyotis chrysotricha.Methods:Microscopy and thin-layer chromatography were used to identify the characteristics of Hedyotis chrysotricha.The contents of water,total ash,acid-insoluble ash and ethanol soluble extracts were determined to accord to the method of Chinese Pharmacopoeia.HPLC was used for the content determination of ursolic acid and oleanolic acid.The determination was performed on Agilent ZORBAX Eclipse XDB-C_(18) column,mobile phase:methanol-0.2%acetic acid solution(88∶12);flow rate:0.8 mL/min.The detection wavelength was set at 210 nm and the temperature was 30℃.Results:Microscopic characteristics are obvious;TLC had clear spots,good resolution and strong specificity.Ursolic acid and oleanolic acid showed good linear relationships within the range of 0.0376 mg/mL~0.9399 mg/mL and 0.0074 mg/mL~0.1845 mg/mL,whose average recoveries were 98.61%and 97.63%with the RSDs of 1.07%and 1.13%,respectively.Total ash of medicinal materials was 11.4%~21.4%,acid insoluble ash was 3.0%~11.9%,water content was 8.4%~11.0%,ursolic acid and oleanolic acid was 1.43~3.41 mg/g.Conclusion:This reasonable and feasible method can be used for the quality control of Hedyotis chrysotricha.

关 键 词：金毛耳草 质量标准 显微鉴别 薄层色谱 高效液相色谱法 熊果酸 齐墩果酸 

分 类 号：TQ[化学工程]