陕西奶牛源微小隐孢子虫的基因亚型鉴定及其遗传进化分析  

Genotyping and Phylogenetic Analysis of Cryptosporidium parvum from Dairy Cows in Shaanxi Province

作　　者：张迎锐 王占宏 丰嘉怡 赵永攀 陈超 李韬 张智鹏 ZHANG Yingrui;WANG Zhanhong;FENG Jiayi;ZHAO Yongpan;CHEN Chao;LI Tao;ZHANG Zhipeng(Mianxian County Center of Animal Disease Control,Mianxian,Shaanxi 724299,China;Yangxian County Center of Animal Disease Control,Yangxian,Shaanxi 724299,China;College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi 712100,China;Shaanxi Animal Husbandry Experiment and Demonstration Center,Jinyang,Shaanxi 713702,China;Hanzhong City Center of Animal Disease Control,Hanzhong,Shaanxi 723099,China)

机构地区：[1]陕西省汉中市勉县动物疫病预防控制中心,陕西勉县724299 [2]陕西省洋县动物疫病预防控制中心,陕西洋县723300 [3]西北农林科技大学动物医学院,陕西杨凌712100 [4]陕西省畜牧产业试验示范中心,陕西泾阳713702 [5]陕西省汉中市动物疫病预防控制中心,陕西汉中723099

出　　处：《家畜生态学报》2024年第10期72-76,共5页Journal of Domestic Animal Ecology

基　　金：银川市2023年推进产学研融合助力农业高质量发展项目(K4050723181)。

摘　　要：隐孢子虫是一种寄生于人和多种动物消化道的人兽共患寄生性原虫,可通过污染食物、水源等感染宿主,常引起宿主水样腹泻,甚至死亡。微小隐孢子虫(Cryptosporidium parvum)是隐孢子虫属中致病力较高的种类之一,有研究表明,C.parvum的致病力与其gp60基因亚型有强相关性。因此,本研究基于gp60基因位点并应用分子生物学方法对分离自陕西奶牛源微小隐孢子虫进行基因亚型鉴定和遗传进化分析。结果表明,该研究中奶牛源C.parvum分离株(SX-1)属于Ⅱd亚型家族,基因亚型为ⅡdA19G1,与GenBank中MH049734、MN304768、MT156342和AY738194等参考分离株同归于一个大支,均属于Ⅱd亚型家族。该研究所获得的结果表明C.parvum在陕西省奶牛中分布广泛且具有遗传多样性,对做好犊牛腹泻疾病的精准防控具有重要意义,可为陕西奶牛源C.parvum有效防控策略的制定提供理论基础和数据支撑。Cryptosporidium,a zoonotic protozoon infecting host animals via the contaminated ingestion of food,water,and other sources,parasitize the digestive tracts of humans and various animals and cause watery diarrhea and even death of infected host.Cryptosporidium parvum is one of the most virulent species in the genus Cryptosporidium,and previous studies also have shown that the virulence of C.parvum have strong relevance to its genotype.Based on gp60 gene locus,this study therefore utilized molecular biological methods to identify subtypes and analyze genetic evolution of C.parvum isolated from dairy cattle in Shaanxi Province.The results showed that C.parvum isolate(SX-1)from dairy cattle in this study was identified as the genotypeⅡdA19G1 belonging to theⅡd subtype family.According to the phylogenetic relationship tree constructed,the SX-1 isolates here was grouped in one separate branch with the MH049734,MN304768,MT156342 and AY738194 isolates in GenBank as the IId subtype family.In conclusion,these findings revealed the wide distribution and genetic diversity of C.parvum in dairy cattle in Shaanxi province,and the significance of developing accurate strategies for the prevention and control of calf diarrhea.Additionally,it provides theoretical basis and data support for the formulation of effective prevention and control strategies for C.parvum from dairy cattle in Shaanxi Province.

关 键 词：微小隐孢子虫 基因亚型 遗传进化分析 奶牛 陕西 

分 类 号：S852.723[农业科学—基础兽医学] S858.23[农业科学—兽医学]