醋酸铅与纳米硫化铅暴露对血脑脊液屏障免疫监视功能损伤的比较研究  

Comparative study on the immune surveillance injury of blood cerebrospinal fluid barrier induced by exposure to lead acetate and nano-lead sulfide

作　　者：冯佩佩 黄炎 张倩影 刘坤 李秀茹 李敏 Feng Peipei;Huang Yan;Zhang Qianying;Liu Kun;Li Xiuru;Li Min(Department of Pharmacy,North China University of Science and Techology Affiliated Hospital,Tangshan 063000,China)

机构地区：[1]华北理工大学附属医院药学部,唐山063000

出　　处：《中华劳动卫生职业病杂志》2024年第9期650-655,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：河北省医学科学研究课题计划(20180757)。

摘　　要：目的探讨比较醋酸铅与纳米硫化铅暴露对大鼠血脑脊液屏障免疫监视功能损伤的差异,为铅和纳米铅暴露致神经损伤的机制研究提供依据。方法于2015年6月,将45只SPF级SD雄性大鼠随机分为对照组、醋酸铅组(20 mg/kg)和纳米硫化铅组(20 mg/kg),每组15只。每周灌胃染毒5次,共9周。采用流式细胞术检测大鼠血液和脑脊液中CD4^(+)T淋巴细胞的数量;运用ELISA法检测大鼠血清和脑脊液中白细胞介素4(IL-4)、干扰素-γ(IFN-γ)的含量;激光扫描共聚焦荧光免疫法检测大鼠脉络丛中细胞间黏附分子-1(ICAM-1)和CD4^(+)T淋巴细胞的表达分布;采用real-time PCR法检测大鼠脉络丛中IL-4、IFN-γ和ICAM-1 mRNA的表达水平。结果与对照组比较,醋酸铅组大鼠血液中CD4^(+)T淋巴细胞所占比例增高,醋酸铅组和纳米硫化铅组大鼠脑脊液中CD4^(+)T淋巴细胞所占比例增高,醋酸铅组和纳米硫化铅组大鼠血清中IL-4和IFN-γ的含量均升高,醋酸铅组大鼠脑脊液中IL-4含量和纳米硫化铅组大鼠脑脊液中IL-4、IFN-γ含量升高,差异均有统计学意义(P<0.05)。醋酸铅组和纳米硫化铅组大鼠脉络丛中ICAM-1及CD4^(+)T淋巴细胞的荧光强度均较对照组增强,纳米硫化铅组CD4^(+)T淋巴细胞的荧光强度较醋酸铅组弱。与对照组比较,醋酸铅组和纳米硫化铅组大鼠脉络丛中ICAM-1、IL-4和IFN-γmRNA的表达水平升高,且纳米硫化铅组ICAM-1和IL-4 mRNA的表达水平高于醋酸铅组,IFN-γmRNA的表达水平低于醋酸铅组(P<0.05)。结论醋酸铅和纳米硫化铅染毒可致大鼠CD4^(+)T淋巴细胞、IL-4、IFN-γ及ICAM-1水平增加,这可能与血脑脊液屏障免疫监视功能受到损伤有关,且醋酸铅与纳米硫化铅致损伤的结果存在差异。Objective To investigate the differences in terms of blood cerebrospinal fluid barrier immune surveillance injury by lead acetate and nano-lead sulfide exposure in order to provide basis for the study of their mechanism of nerve injury caused by exposure to lead and nano lead.Methods In June 2015,forty-five SPF SD male rats were randomly divided into control group,lead acetate group(20 mg/kg)and nano-lead sulfide group(20 mg/kg),with 15 rats in each group.The rats were intragastric five times a week,for nine weeks.The numbers of CD4^(+)T lymphocytes in blood and cerebrospinal fluid were detected by flow cytometry.The levels of interleukin-4(IL-4)and interferon-γ(IFN-γ)in serum and cerebrospinal fluid were detected by ELISA.The expressions and distribution of intercellular cell adhesion molecule-1(ICAM-1)and CD4^(+)T lymphocytes in choroid plexus were detected by laser confocal fluorescence immunoassay.The mRNA expression levels of IL-4,IFN-γand ICAM-1 in the choroid plexus were detected by real-time PCR.Results Compared with the control group,the proportion of CD4^(+)T lymphocytes in blood of rats in lead acetate group was increased,the proportions of CD4^(+)T lymphocytes in cerebrospinal fluid of rats in lead acetate group and nano-lead sulfide group were increased,the contents of IL-4 and IFN-γin serum of rats in lead acetate group and nano-lead sulfide group were increased,the content of IL-4 in cerebrospinal fluid of rats in lead acetate group and the contents of IL-4 and IFN-γin cerebrospinal fluid of rats in nano-lead sulfide group were increased,the differences were statistically significant(P<0.05).The fluorescence intensity of ICAM-1 and CD4^(+)T lymphocytes in choriochoroid plexus of rats in lead acetate group and nano-lead sulfide group were stronger than those in control group,and the fluorescence intensity of CD4^(+)T lymphocytes of rats in nano-lead sulfide group was weaker than that in lead acetate group.Compared with the control group,the mRNA expression levels of ICAM-1,IL-4 and IFN-γin cho

关 键 词：铅 纳米硫化铅 血脑脊液屏障 免疫监视功能 

分 类 号：R392[医药卫生—免疫学]