Lens-free on-chip 3D microscopy based on wavelength-scanning Fourier ptychographic diffraction tomography  

作　　者：Xuejuan Wu Ning Zhou Yang Chen Jiasong Sun Linpeng Lu Qian Chen Chao Zuo 

机构地区：[1]Smart Computational Imaging(SCI)Laboratory,Nanjing University of Science and Technology,No.200 Xiaolingwei Street,210094 Nanjing,Jiangsu,China [2]Smart Computational Imaging Research Institute(SCIRI)of Nanjing University of Science and Technology,210094 Nanjing,Jiangsu,China [3]Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense,No.200 Xiaolingwei Street,210094 Nanjing,Jiangsu,China

出　　处：《Light(Science & Applications)》2024年第9期1964-1979,共16页光（科学与应用)（英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFA1205002,2024YFE0101300);National Natural Science Foundation of China(62105151,62175109,U21B2033,62227818,62361136588);Leading Technology of Jiangsu Basic Research Plan(BK20192003);Youth Foundation of Jiangsu Province(BK20210338);Biomedical Competition Foundation of Jiangsu Province(BE2022847);Key National Industrial Technology Cooperation Foundation of Jiangsu Province(BZ2022039);Fundamental Research Funds for the Central Universities(30920032101,30923010206);Fundamental Scientific Research Business Fee Funds for the Central Universities(2023102001);Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense(JSGP202105,JSGP202201).

摘　　要：Lens-free on-chip microscopy is a powerful and promising high-throughput computational microscopy technique due to its unique advantage of creating high-resolution images across the full field-of-view(FOV)of the imaging sensor.Nevertheless,most current lens-free microscopy methods have been designed for imaging only two-dimensional thin samples.Lens-free on-chip tomography(LFOCT)with a uniform resolution across the entire FOV and at a subpixel level remains a critical challenge.In this paper,we demonstrated a new LFOCT technique and associated imaging platform based on wavelength scanning Fourier ptychographic diffraction tomography(wsFPDT).Instead of using angularlyvariable illuminations,in wsFPDT,the sample is illuminated by on-axis wavelength-variable illuminations,ranging from 430 to 1200 nm.The corresponding under-sampled diffraction patterns are recorded,and then an iterative ptychographic reconstruction procedure is applied to fill the spectrum of the three-dimensional(3D)scattering potential to recover the sample’s 3D refractive index(RI)distribution.The wavelength-scanning scheme not only eliminates the need for mechanical motion during image acquisition and precise registration of the raw images but secures a quasi-uniform,pixel-super-resolved imaging resolution across the entire imaging FOV.With wsFPDT,we demonstrate the high-throughput,billion-voxel 3D tomographic imaging results with a half-pitch lateral resolution of 775 nm and an axial resolution of 5.43μm across a large FOV of 29.85mm2 and an imaging depth of>200μm.The effectiveness of the proposed method was demonstrated by imaging various types of samples,including micropolystyrene beads,diatoms,and mouse mononuclear macrophage cells.The unique capability to reveal quantitative morphological properties,such as area,volume,and sphericity index of single cell over large cell populations makes wsFPDT a powerful quantitative and label-free tool for high-throughput biological applications.

关 键 词：free ILLUMINATION eliminate 

分 类 号：O43[机械工程—光学工程]