兔病毒性出血症二价口服疫苗免疫抗体水平测定  

Determination of Immune Antibody Levels in Bivalent Oral Vaccine Against Rabbit Viral Hemorrhagic Disease

作　　者：任永军[1,2] 杨泽晓 袁定胜[1,2] 邝良德[1,2] REN Yongjun;YANG Zexiao;YUAN Dingsheng;KUANG Liangde(Sichuan Animal Science Academy,chengdu 610066;Animal Breeding and Genetics key Laboratory of Sichuan Province,Chengdu 610066;College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611303)

机构地区：[1]四川省畜牧科学研究院,成都610066 [2]动物遗传育种四川省重点实验室,成都610066 [3]四川农业大学动物医学院,成都611303

出　　处：《中国养兔》2024年第4期18-20,24,共4页Chinese Journal of Rabbit Farming

基　　金：兔病毒性出血症二价口服疫苗技术成果引进与示范推广(2023ZHYZ0006);油樟油绿色饲料产品开发关键技术研究与示范(2023YFN0076);十四五育种攻关项目:优质肉兔配套系选育(2021YFYZ0033);财政专项:肉兔配套系培育及地方遗传资源的保护利用研究(SASA2023CZYX004)。

摘　　要：为验证兔病毒性出血症二价口服疫苗(AN301,R301-1)饮水免疫的免疫效果,分别选择母兔和商品兔进行了饮水免疫1次,取免疫后7、14、21、28、42 d等时间点采集血清样品,并按照一定浓度梯度稀释后分别采用间接ELISA和阻断ELISA进行了RHDV1和RHDV2的抗体水平检测。结果显示,受试母兔免疫后14、28 d、产仔后7和14 d期间RHDV1和RHDV2抗体效价都在1∶1000~1∶8000之间,抗体水平增加并趋于稳定;商品兔免疫7、14、28、42 d期间RHDV1和RHDV2抗体水平都在1∶1000~1∶8000之间,抗体水平增加并趋于稳定。说明兔病毒性出血症二价口服疫苗(AN301,R301-1)饮水免疫后可刺激母兔和商品兔产生相对稳定的RHDV1和RHDV2的抗体水平,为该疫苗的进一步推广应用提供了科学参考。To verify the effect of the RHD bivalent oral vaccine(AN301,R301-1)induced by drinking immunolo-gy,Female rabbits and commercial rabbits were respectively immunized with R301-1 once,Collect serum sam-ples at time points 7,14,21,28,and 42 d after immunization,After dilution according to a certain concentration gradient,the antibody levels of RHDV1 and RHDV2 were detected using indirect ELISA and blocking ELISA,re-spectively.The results showed that the titers of RHDV1 and RHDV2 antibodies were between 1:1000 and 1:8000 during the 14th,to postpartum 14 d after immunization of the female rabbits,and the antibody levels increased and tended to stabilize;During the immunization period of 7 to 42d for commercial rabbits after immunization,the lev-els of RHDV1 and RHDV2 antibodies were all between 1:1000 and 1:8000,and the antibody levels increased and tended to stabilize,too.This indicates that the bivalent oral vaccine(AN301,R301-1)for rabbit viral hemor-rhagic disease can stimulate the production of relatively stable antibody levels of RHDV1 and RHDV2 in both fe-male and commercial rabbits after water immunization,this study provided scientific reference for the further pro-motion and application of the vaccine.

关 键 词：RHDV1 RHDV2 二价口服疫苗 

分 类 号：S829.1[农业科学—畜牧学]